論文 - 詳細
| RRC ID | 64398 |
|---|---|
| 著者 | Muramatsu-Maekawa Y, Kawakami K, Fujita Y, Takai M, Kato D, Nakane K, Kato T, Tsuchiya T, Koie T, Miura Y, Ito M, Mizutani K. |
| タイトル | Profiling of Serum Extracellular Vesicles Reveals miRNA-4525 as a Potential Biomarker for Advanced Renal Cell Carcinoma. |
| ジャーナル | Cancer Genomics Proteomics |
| Abstract |
AIM:To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). MATERIALS AND METHODS:EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. RESULTS:Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. CONCLUSION:These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC. |
| 巻・号 | 18(3) |
| ページ | 253-259 |
| 公開日 | 2021-1-1 |
| DOI | 10.21873/cgp.20256 |
| PII | 18/3/253 |
| PMID | 33893078 |
| PMC | PMC8126334 |
| MeSH | Adult Biomarkers, Tumor / blood Carcinoma, Renal Cell / blood* Carcinoma, Renal Cell / genetics Carcinoma, Renal Cell / pathology Extracellular Vesicles / metabolism* Female Humans Kidney Neoplasms / blood* Kidney Neoplasms / genetics Kidney Neoplasms / pathology Male MicroRNAs / blood* MicroRNAs / genetics Middle Aged Transfection |
| IF | 3.28 |
| リソース情報 | |
| ヒト・動物細胞 | OS-RC-2(RCB0735) |