RRC ID 64416
Author Heijo H, Shimogama S, Nakano S, Miyata A, Iwao Y, Hara Y.
Title DNA content contributes to nuclear size control in Xenopus laevis.
Journal Mol Biol Cell
Abstract Cells adapt to drastic changes in genome quantity during evolution and cell division by adjusting the nuclear size to exert genomic functions. However, the mechanism by which DNA content within the nucleus contributes to controlling the nuclear size remains unclear. Here, we experimentally evaluated the effects of DNA content by utilizing cell-free Xenopus egg extracts and imaging of in vivo embryos. Upon manipulation of DNA content while maintaining cytoplasmic effects constant, both plateau size and expansion speed of the nucleus correlated highly with DNA content. We also found that nuclear expansion dynamics was altered when chromatin interaction with the nuclear envelope or chromatin condensation was manipulated while maintaining DNA content constant. Furthermore, excess membrane accumulated on the nuclear surface when the DNA content was low. These results clearly demonstrate that nuclear expansion is determined not only by cytoplasmic membrane supply but also by the physical properties of chromatin, including DNA quantity and chromatin structure within the nucleus, rather than the coding sequences themselves. In controlling the dynamics of nuclear expansion, we propose that chromatin interaction with the nuclear envelope plays a role in transmitting chromatin repulsion forces to the nuclear membrane.
Volume 31(24)
Pages 2703-2717
Published 2020-11-15
DOI 10.1091/mbc.E20-02-0113
PMID 32997613
PMC PMC7927187
MeSH Animals Cell Nucleus / metabolism Cell Nucleus Size / genetics* Cell Nucleus Size / physiology Chromatin / metabolism Chromatin / physiology* Chromosomes / genetics Cytosol / metabolism DNA / chemistry DNA / metabolism* DNA Replication / genetics Nuclear Envelope / physiology Oocytes / metabolism Ovum / physiology Xenopus Proteins / genetics Xenopus laevis / genetics
IF 3.791
Clawed frogs / Newts