RRC ID 6442
Author Zhang Y, Yan L, Zhou Z, Yang P, Tian E, Zhang K, Zhao Y, Li Z, Song B, Han J, Miao L, Zhang H.
Title SEPA-1 mediates the specific recognition and degradation of P granule components by autophagy in C. elegans.
Journal Cell
Abstract How autophagy, an evolutionarily conserved intracellular catabolic system for bulk degradation, selectively degrades protein aggregates is poorly understood. Here, we show that several maternally derived germ P granule components are selectively eliminated by autophagy in somatic cells during C. elegans embryogenesis. The activity of sepa-1 is required for the degradation of these P granule components and for their accumulation into aggregates, termed PGL granules, in autophagy mutants. SEPA-1 forms protein aggregates and is also a preferential target of autophagy. SEPA-1 directly binds to the P granule component PGL-3 and also to the autophagy protein LGG-1/Atg8. SEPA-1 aggregates consistently colocalize with PGL granules and with LGG-1 puncta. Thus, SEPA-1 functions as a bridging molecule in mediating the specific recognition and degradation of P granule components by autophagy. Our study reveals a mechanism for preferential degradation of protein aggregates by autophagy and emphasizes the physiological significance of selective autophagy during animal development.
Volume 136(2)
Pages 308-21
Published 2009-1-23
DOI 10.1016/j.cell.2008.12.022
PII S0092-8674(08)01614-0
PMID 19167332
MeSH Amino Acid Sequence Animals Autophagy* Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / chemistry Carrier Proteins / genetics Carrier Proteins / metabolism* Cytoplasmic Granules / metabolism* Disorders of Sex Development Extrachromosomal Inheritance Male Molecular Sequence Data RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism
IF 38.637
Times Cited 143
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1642 tm2976