RRC ID 64444
Author Azuma N, Omachi S, Hanazawa W, Morimoto A, Sanjoba C, Matsumoto Y, Fujii W, Goto Y.
Title Variable dependency on BAFF in IgG antibody production during Leishmania infection.
Journal Parasitol Int
Abstract B-cell activating factor (BAFF) is known as a cytokine responsible for survival and activation of B cells. However, involvement of the molecule in IgG antibody production during infection remains elusive. In this study, dependency of antibody production in Leishmania infection on BAFF was examined by using BAFF-knockout (BAFF-KO) mice. When BAFF-KO mice were infected with L. major, there was no significant difference in lesion development or parasite burden from those in infected wildtype mice. In contrast, levels of IgG antibodies to Leishmania crude antigen were lower in BAFF-KO mice, suggesting that antibody production during L. major infection is BAFF-dependent. ELISA using defined leishmanial antigens demonstrated that the influence of BAFF on antibody production during L. major varies depending on antigens; IgG production to tandem repeat proteins were more affected by BAFF than non-repeat antigens. On the contrary, all of the defined antigens tested were strongly affected by BAFF for IgG antibody production during L. donovani infection. These results suggest degree of BAFF contribution to antibody production during infection is variable depending on the type of infection and even on the type of antigen in a given infection. These results may explain contradictory roles of BAFF in antibody production in previous works.
Volume 74
Pages 101997
Published 2020-2-1
DOI 10.1016/j.parint.2019.101997
PII S1383-5769(19)30348-4
PMID 31626986
MeSH Animals Antibodies, Protozoan / immunology* Antigens, Protozoan / immunology B-Cell Activating Factor / genetics B-Cell Activating Factor / immunology* Female Immunoglobulin G / immunology* Leishmania donovani / immunology Leishmaniasis / immunology* Mice Mice, Inbred BALB C Mice, Knockout
IF 1.866
Pathogenic eukaryotic microorganisms Leishmania donovani D10 (Ld003)