| RRC ID |
6445
|
| Author |
Claycomb JM, Batista PJ, Pang KM, Gu W, Vasale JJ, van Wolfswinkel JC, Chaves DA, Shirayama M, Mitani S, Ketting RF, Conte D Jr, Mello CC.
|
| Title |
The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation.
|
| Journal |
Cell
|
| Abstract |
RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the RNA-dependent RNA polymerase EGO-1, the Dicer-related helicase DRH-3, and the Tudor-domain protein EKL-1 localize to chromosomes and are required for proper chromosome segregation. In the absence of these factors chromosomes fail to align at the metaphase plate and kinetochores do not orient to opposing spindle poles. Surprisingly, the CSR-1-interacting small RNAs (22G-RNAs) are antisense to thousands of germline-expressed protein-coding genes. Nematodes assemble holocentric chromosomes in which continuous kinetochores must span the expressed domains of the genome. We show that CSR-1 interacts with chromatin at target loci but does not downregulate target mRNA or protein levels. Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans.
|
| Volume |
139(1)
|
| Pages |
123-34
|
| Published |
2009-10-2
|
| DOI |
10.1016/j.cell.2009.09.014
|
| PII |
S0092-8674(09)01174-X
|
| PMID |
19804758
|
| PMC |
PMC2766185
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism*
Chromosome Segregation*
DEAD-box RNA Helicases / metabolism
RNA-Dependent RNA Polymerase / metabolism
|
| IF |
38.637
|
| Times Cited |
234
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1599
tm892 |
