| RRC ID |
64496
|
| 著者 |
Kamal N, Mun T, Reid D, Lin JS, Akyol TY, Sandal N, Asp T, Hirakawa H, Stougaard J, Mayer KFX, Sato S, Andersen SU.
|
| タイトル |
Insights into the evolution of symbiosis gene copy number and distribution from a chromosome-scale Lotus japonicus Gifu genome sequence.
|
| ジャーナル |
DNA Res
|
| Abstract |
Lotus japonicus is a herbaceous perennial legume that has been used extensively as a genetically tractable model system for deciphering the molecular genetics of symbiotic nitrogen fixation. Our aim is to improve the L. japonicus reference genome sequence, which has so far been based on Sanger and Illumina sequencing reads from the L. japonicus accession MG-20 and contained a large fraction of unanchored contigs. Here, we use long PacBio reads from L. japonicus Gifu combined with Hi-C data and new high-density genetic maps to generate a high-quality chromosome-scale reference genome assembly for L. japonicus. The assembly comprises 554 megabases of which 549 were assigned to six pseudomolecules that appear complete with telomeric repeats at their extremes and large centromeric regions with low gene density. The new L. japonicus Gifu reference genome and associated expression data represent valuable resources for legume functional and comparative genomics. Here, we provide a first example by showing that the symbiotic islands recently described in Medicago truncatula do not appear to be conserved in L. japonicus.
|
| 巻・号 |
27(3)
|
| 公開日 |
2020-6-1
|
| DOI |
10.1093/dnares/dsaa015
|
| PII |
5870829
|
| PMID |
32658273
|
| PMC |
PMC7508351
|
| MeSH |
Chromosomes*
Gene Dosage*
Gene Expression Regulation, Plant
Genes, Plant
Genomics
Lotus / genetics*
Medicago / genetics
Medicago truncatula / genetics
Nitrogen Fixation
Symbiosis / genetics*
|
| IF |
4.009
|
| リソース情報 |
| ミヤコグサ・ダイズ |
Lotus japonicus Gifu reference genome |
