論文 - 詳細
| RRC ID | 64505 |
|---|---|
| 著者 | Zappia MP, de Castro L, Ariss MM, Jefferson H, Islam AB, Frolov MV. |
| タイトル | A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence in Drosophila. |
| ジャーナル | EMBO Rep |
| Abstract |
In Drosophila, the wing disc-associated muscle precursor cells give rise to the fibrillar indirect flight muscles (IFM) and the tubular direct flight muscles (DFM). To understand early transcriptional events underlying this muscle diversification, we performed single-cell RNA-sequencing experiments and built a cell atlas of myoblasts associated with third instar larval wing disc. Our analysis identified distinct transcriptional signatures for IFM and DFM myoblasts that underlie the molecular basis of their divergence. The atlas further revealed various states of differentiation of myoblasts, thus illustrating previously unappreciated spatial and temporal heterogeneity among them. We identified and validated novel markers for both IFM and DFM myoblasts at various states of differentiation by immunofluorescence and genetic cell-tracing experiments. Finally, we performed a systematic genetic screen using a panel of markers from the reference cell atlas as an entry point and found a novel gene, Amalgam which is functionally important in muscle development. Our work provides a framework for leveraging scRNA-seq for gene discovery and details a strategy that can be applied to other scRNA-seq datasets. |
| 巻・号 | 21(10) |
| ページ | e49555 |
| 公開日 | 2020-10-5 |
| DOI | 10.15252/embr.201949555 |
| PMID | 32815271 |
| PMC | PMC7534622 |
| MeSH | Animals Drosophila* / genetics Drosophila Proteins* / genetics Drosophila melanogaster / genetics Muscle Development / genetics Wings, Animal |
| IF | 7.497 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#103970 DGRC#110692 DGRC#110626 DGRC#102845 |