RRC ID 64514
Author Pridie C, Simmonds AJ.
Title The role of Peroxin 7 during Drosophila embryonic development.
Journal Genome
Abstract Peroxisomes are organelles in eukaryotic cells responsible for processing several types of lipids and management of reactive oxygen species. A conserved family of peroxisome biogenesis (Peroxin, Pex) genes encode proteins essential to peroxisome biogenesis or function. In yeast and mammals, PEROXIN7 (PEX7) acts as a cytosolic receptor protein that targets enzymes containing a peroxisome targeting signal 2 (PTS2) motif for peroxisome matrix import. The PTS2 motif is not present in the Drosophila melanogaster homologs of these enzymes. However, the fly genome contains a Pex7 gene (CG6486) that is very similar to yeast and human PEX7. We find that Pex7 is expressed in tissue-specific patterns analogous to differentiating neuroblasts in D. melanogaster embryos. This is correlated with a requirement for Pex7 in this cell lineage as targeted somatic Pex7 knockout in embryonic neuroblasts reduced survival. We also found that Pex7 over-expression in the same cell lineages caused lethality during the larval stage. Targeted somatic over-expression of a Pex7 transgene in neuroblasts of Pex7 homozygous null mutants resulted in a semi-lethal phenotype similar to targeted Pex7 knockout. These findings suggest that D. melanogaster has tissue-specific requirements for Pex7 during embryo development.
Volume 64(2)
Pages 119-137
Published 2021-2-1
DOI 10.1139/gen-2019-0207
PMID 32191843
MeSH Animals Drosophila melanogaster* / embryology Drosophila melanogaster* / genetics Gene Expression Regulation, Developmental Neurons / cytology* Peroxisomal Targeting Signal 2 Receptor* / metabolism Protein Transport Receptors, Cytoplasmic and Nuclear Saccharomyces cerevisiae
IF 2.037
Drosophila DGRC#206194