論文 - 詳細
| RRC ID | 64514 |
|---|---|
| 著者 | Pridie C, Simmonds AJ. |
| タイトル | The role of Peroxin 7 during Drosophila embryonic development. |
| ジャーナル | Genome |
| Abstract |
Peroxisomes are organelles in eukaryotic cells responsible for processing several types of lipids and management of reactive oxygen species. A conserved family of peroxisome biogenesis (Peroxin, Pex) genes encode proteins essential to peroxisome biogenesis or function. In yeast and mammals, PEROXIN7 (PEX7) acts as a cytosolic receptor protein that targets enzymes containing a peroxisome targeting signal 2 (PTS2) motif for peroxisome matrix import. The PTS2 motif is not present in the Drosophila melanogaster homologs of these enzymes. However, the fly genome contains a Pex7 gene (CG6486) that is very similar to yeast and human PEX7. We find that Pex7 is expressed in tissue-specific patterns analogous to differentiating neuroblasts in D. melanogaster embryos. This is correlated with a requirement for Pex7 in this cell lineage as targeted somatic Pex7 knockout in embryonic neuroblasts reduced survival. We also found that Pex7 over-expression in the same cell lineages caused lethality during the larval stage. Targeted somatic over-expression of a Pex7 transgene in neuroblasts of Pex7 homozygous null mutants resulted in a semi-lethal phenotype similar to targeted Pex7 knockout. These findings suggest that D. melanogaster has tissue-specific requirements for Pex7 during embryo development. |
| 巻・号 | 64(2) |
| ページ | 119-137 |
| 公開日 | 2021-2-1 |
| DOI | 10.1139/gen-2019-0207 |
| PMID | 32191843 |
| MeSH | Animals Drosophila melanogaster* / embryology Drosophila melanogaster* / genetics Gene Expression Regulation, Developmental Neurons / cytology* Peroxisomal Targeting Signal 2 Receptor* / metabolism Protein Transport Receptors, Cytoplasmic and Nuclear Saccharomyces cerevisiae |
| IF | 2.037 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#206194 |