論文 - 詳細
| RRC ID | 64524 |
|---|---|
| 著者 | Ohkubo Y, Kuwata K, Matsubayashi Y. |
| タイトル | A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1. |
| ジャーナル | Nat Plants |
| Abstract |
The nitrate transporter NRT2.1, which plays a central role in high-affinity nitrate uptake in roots, is activated at the post-translational level in response to nitrogen (N) starvation1,2. However, the critical enzymes required for the post-translational activation of NRT2.1 remain to be identified. Here, we show that a type 2C protein phosphatase, designated CEPD-induced phosphatase (CEPH), activates high-affinity nitrate uptake by directly dephosphorylating Ser501 of NRT2.1, a residue that functions as a negative phospho-switch in Arabidopsis2. CEPH is predominantly expressed in epidermal and cortex cells in roots and is upregulated by N starvation via a CEPDL2/CEPD1/2-mediated long-distance signalling from shoots3,4. The loss of CEPH leads to marked decreases in high-affinity nitrate uptake, tissue nitrate content and plant biomass. Collectively, our results identify CEPH as a crucial enzyme in the N-starvation-dependent activation of NRT2.1 and provide molecular and mechanistic insights into how plants regulate high-affinity nitrate uptake at the post-translational level in response to the N environment. |
| 巻・号 | 7(3) |
| ページ | 310-316 |
| 公開日 | 2021-3-1 |
| DOI | 10.1038/s41477-021-00870-9 |
| PII | 10.1038/s41477-021-00870-9 |
| PMID | 33686225 |
| MeSH | Anion Transport Proteins / metabolism* Arabidopsis / enzymology Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Glutaredoxins / metabolism Nitrates / metabolism* Phosphorylation Serine / metabolism |
| IF | 13.256 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 29 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst14870 |