RRC ID 64529
Author Dean GH, Pang F, Haughn GW, Kunst L.
Title A simple, non-toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants.
Journal Appl Plant Sci
Abstract Premise:Seed oil is an economically important trait in Brassica oilseed crops. A novel method was developed to isolate Arabidopsis thaliana seeds with altered oil content.
Methods and Results:In A. thaliana, seed oil content is correlated with seed density, with high-oil seeds being less dense than wild type and tending to float in solution, and low-oil seeds being denser and tending to sink. In contrast to previous methods, which used toxic chemicals and density gradient centrifugation, different concentrations of calcium chloride (CaCl2) were employed to separate seeds without the need for centrifugation. The method was validated using known seed oil mutants, and 120,822 T-DNA mutagenized A. thaliana lines were then screened for novel seed density phenotypes.
Conclusions:A number of candidate mutants, as well as new alleles of two genes known to influence seed oil biosynthesis, were successfully isolated.
Volume 8(4)
Pages e11332
Published 2020-4-1
DOI 10.1002/aps3.11332
PII APS311332
PMID 32351794
PMC PMC7186901
IF 1.591
Arabidopsis / Cultured plant cells, genes pss