Reference - Detail
| RRC ID | 64530 |
|---|---|
| Author | Tanaka K, Miyazawa M, Mikami M, Aoki D, Kiguchi K, Iwamori M. |
| Title | Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines. |
| Journal | Glycoconj J |
| Abstract |
Monoclonal antibody YHD-06 generated by immunization with GM2 reacted with gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, among which GalNAc-GD1a was characterized as an antigen of autoimmune peripheral neuropathies including Guillain-Barré syndrome. When glycolipids were examined by TLC-immunostaining with YHD-06 in seven human cervical carcinoma-derived cell lines, GM2 was found in all cell lines, amounting to 15.5 % to 57.5 % of total gangliosides. Whereas GalNAc-GD1a was present in three cell lines, amounting to 5.4-17.5 % of total gangliosides, and GalNAc-GM1b in four cell lines in amounts of less than 2 %. The elevated amounts of gangliosides with GM2 determinant were closely correlated with the relative intensities of gene expression of GalNAc transferase, this being characteristic of cervical carcinoma-derived cells. However, in tissues from patients with several histological types of cervical carcinomas, GM3 was ubiquitously expressed in amounts of more than 66 % of total gangliosides, GM2 was expressed in only five of 15 tissues, and both GalNAc-GM1b and GalNAc-GD1a were not even detected in trace amounts. Since GM1 was detected in all tissues in amounts of less than 0.06 μg/mg dried tissue, all cervical carcinoma tissues were revealed to exhibit GM2 synthesis, indicating that enhanced synthesis of gangliosides with GM2 determinant is a characteristic of cultivated cells in vitro. Similarly, although I(3)SO3-GalCer was not present in the squamous cell carcinoma (SCC) tissues, SCC-derived cells selectively expressed II(3)SO3-LacCer. Since enhanced synthesis of GM2 has been reported in SV-40 virus-transfected fibroblasts, papilloma virus might be involved in the expression of GM2 in cervical carcinoma-derived cells. |
| Volume | 33(5) |
| Pages | 745-54 |
| Published | 2016-10-1 |
| DOI | 10.1007/s10719-016-9668-0 |
| PII | 10.1007/s10719-016-9668-0 |
| PMID | 27270764 |
| MeSH | Female G(M2) Ganglioside / biosynthesis* Gene Expression Regulation, Neoplastic* HeLa Cells Humans Uterine Cervical Neoplasms / metabolism* |
| IF | 2.197 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | HeLa |