論文 - 詳細
| RRC ID | 6454 |
|---|---|
| 著者 | Maekawa M, Inoue T, Kobuna H, Nishimura T, Gengyo-Ando K, Mitani S, Arai H. |
| タイトル | Functional analysis of GS28, an intra-Golgi SNARE, in Caenorhabditis elegans. |
| ジャーナル | Genes Cells |
| Abstract |
Intra-Golgi retrograde transport is assumed to maintain Golgi function by recycling Golgi-resident proteins to younger cisternae in the progression of entire Golgi stack from cis to trans. GS28 (Golgi SNARE of 28 kDa, also known as GOS28) is a Golgi-localized SNARE protein and has been implicated in intra-Golgi retrograde transport. However, the in vivo functions of GS28, and consequently, the roles of the intra-Golgi retrograde transport in animal development are largely unknown. In this study, we generated deletion mutants of Caenorhabditis elegans GS28 and performed a synthetic lethal RNAi screen using GS28 mutants. We found that another Golgi-localized SNARE, Ykt6, functions cooperatively with GS28 in embryonic development. During post-embryonic development, GS28 mutants exhibited reduced seam cell numbers and a missing ray phenotype under Ykt6 knockdown conditions, suggesting that cell proliferation and/or differentiation of stem cell-like seam cells are impaired in GS28- and Ykt6-depleted worms. We also demonstrated that GS28 and Ykt6 act redundantly for the proper expression of Golgi-resident proteins in adult intestinal cells. This study reveals the in vivo importance of the Golgi-localized SNAREs GS28 and Ykt6. |
| 巻・号 | 14(8) |
| ページ | 1003-13 |
| 公開日 | 2009-8-1 |
| DOI | 10.1111/j.1365-2443.2009.01325.x |
| PII | GTC1325 |
| PMID | 19624756 |
| MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Cell Proliferation Epithelial Cells / cytology Gene Deletion Gene Expression Regulation, Developmental* Golgi Apparatus / metabolism* Humans Mice R-SNARE Proteins / genetics R-SNARE Proteins / metabolism* RNA Interference SNARE Proteins / genetics SNARE Proteins / metabolism* Stem Cells / cytology |
| IF | 1.655 |
| 引用数 | 11 |
| WOS 分野 | GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2800 tm3575 |