| RRC ID |
64543
|
| Author |
Zhang D, Tang D, Yamamoto T, Kato Y, Horiuchi S, Ogawa S, Yoshimura E, Suzuki M.
|
| Title |
Improving biosynthesis of AuPd core-shell nanoparticles through Escherichia coli with the assistance of phytochelatin for catalytic enhanced chemiluminescence and benzyl alcohol oxidation.
|
| Journal |
J Inorg Biochem
|
| Abstract |
In this work, AuPd core-shell nanoparticles (NPs) biosynthesized through Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli (Au-Pd/AtPCS1-E. coli) with catalytic enhanced chemiluminescence (CL) and benzyl alcohol oxidation (BAO) was investigated. Such biosynthesis of AuPd core-shell NPs was obviously enhanced due to insertion of the gene sequence of Arabidopsis thaliana phytochelatin synthase (AtPCS1) to a plasmid vector (pET-28b) of Escherichia coli (E. coli). The obtained Arabidopsis thaliana phytochelatin synthase-modified Escherichia coli (AtPCS1-E. coli) could generate phytochelatins (PCs, (γ-Glu-Cys)n-Gly, n > 1) for efficient capture and enrichment of Au3+. The component and morphology of AuPd core-shell NPs were checked through X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM) and energy dispersive spectrometer (EDS). Catalytic CL (in H2O2-luminol system) and BAO (in H2O2-benzyl alcohol system) effect with different experimental conditions were examined, respectively. These results revealed that multifunctional PCs could effectively facilitate biosynthetic process of AuPd core-shell NPs with better distribution, higher yield and lower cost while stronger CL intensity and higher conversion could be obtained for further quantitative analysis and application.
|
| Volume |
199
|
| Pages |
110795
|
| Published |
2019-10-1
|
| DOI |
10.1016/j.jinorgbio.2019.110795
|
| PII |
S0162-0134(19)30311-3
|
| PMID |
31400604
|
| MeSH |
Arabidopsis / metabolism
Benzyl Alcohol / chemistry*
Catalysis
Escherichia coli / metabolism*
Gold / chemistry*
Luminescence
Microscopy, Electron, Scanning
Nanoparticles / chemistry*
Nanoparticles / metabolism*
Oxidation-Reduction
Palladium / chemistry*
Phytochelatins / chemistry
Phytochelatins / metabolism
X-Ray Diffraction
|
| IF |
3.212
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda00376 |
