Reference - Detail
RRC ID | 64558 |
---|---|
Author | Nakayama M, Nakamura A, Hondou T, Miyata H. |
Title | Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field. |
Journal | Int J Radiat Biol |
Abstract |
PURPOSE:Synergistic effects between cellular oxidative stress and magnetic fields may explain the adverse biological effects of 50/60 Hz magnetic fields. To determine whether this hypothesis holds in macrophage RAW264 cells, we measured DNA single-strand breaks (SSB), cell viability, and nitric oxide (NO) production in cells with or without exposure to 0.5-mT, 50-Hz magnetic fields for 24 h and with or without simultaneous stimulation via the bacterial endotoxin, lipopolysaccharide (LPS). MATERIALS AND METHODS:Macrophages stimulated with 10 ng/ml LPS for 1 h were exposed to or not exposed to a magnetic field and were then subjected to (1) the alkaline comet assay to measure SSBs, (2) trypan-blue exclusion assay for cell viability, and (3) measurements of NO for evaluation of oxidative stress. RESULTS:The 50-Hz magnetic field enhanced DNA SSB and decreased cell viability only in the LPS-stimulated macrophages in which NO production was greatly enhanced. The magnetic field alone did not alter NO production. CONCLUSION:Co-stimulation of the cell with LPS and a 50-Hz magnetic field promoted SSB and lowered cell viability, but these were not mediated by LPS-induced NO production. |
Volume | 92(10) |
Pages | 583-9 |
Published | 2016-10-1 |
DOI | 10.1080/09553002.2016.1206224 |
PMID | 27430265 |
MeSH | Animals Cell Survival / drug effects* Cell Survival / radiation effects* DNA Damage / physiology* Dose-Response Relationship, Radiation Electricity Electromagnetic Fields* Lipopolysaccharides / administration & dosage Mice Nitric Oxide / metabolism* Oxidative Stress / drug effects Oxidative Stress / physiology* Oxidative Stress / radiation effects RAW 264.7 Cells Radiation Dosage |
IF | 2.368 |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) |