Reference - Detail
| RRC ID | 64640 |
|---|---|
| Author | Sumiya YU, Inoue T, Ishikawa M, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T. |
| Title | Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Macrophages are important components of human defense systems and consequently key to antitumor immunity. Human-serum macrophage activation factor (serum MAF) can activate macrophages, making it a promising reagent for anticancer therapy. MATERIALS AND METHODS:We established four different macrophage subtypes through introduction of different culture conditions to THP-1- and U937-derived macrophages. We assessed phagocytic activity to understand subtype responses to typical macrophage activation factors (MAFs) and the activation mechanisms of serum MAF. RESULTS:All four macrophage subtypes differed in their response to all MAFs. Moreover, serum MAF had two different activation mechanisms: N-acetylgalactosamine (GalNAc)-dependent and GalNAc-independent. CONCLUSION:Macrophage activation states and mechanisms are heterogeneous. |
| Volume | 36(7) |
| Pages | 3619-23 |
| Published | 2016-7-1 |
| PII | 36/7/3619 |
| PMID | 27354632 |
| MeSH | Humans Lipopolysaccharides / pharmacology Macrophage Activation* Macrophage-Activating Factors / pharmacology* Macrophage-Activating Factors / physiology Macrophages / drug effects Macrophages / physiology* U937 Cells |
| IF | 1.994 |
| Resource | |
| Human and Animal Cells | U937 THP-1(RCB1189) |