RRC ID 64657
Author Saito Y, Kunitomi A, Seki T, Tohyama S, Kusumoto D, Takei M, Kashimura S, Hashimoto H, Yozu G, Motoda C, Shimojima M, Egashira T, Oda M, Fukuda K, Yuasa S.
Title Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells.
Journal FEBS Lett
Abstract The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1, Zfp42, and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3. ES cells containing a triple knockout in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b showed correlation between the fluctuation of Pou5f1, Zfp42, and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.
Volume 591(18)
Pages 2879-2889
Published 2017-9-1
DOI 10.1002/1873-3468.12791
PMID 28805244
MeSH Animals Embryonic Stem Cells / metabolism* Epigenomics Gene Expression / genetics Mice Nanog Homeobox Protein / genetics Octamer Transcription Factor-3 / genetics Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors / genetics
IF 3.057
Human and Animal Cells Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)(AES0146)