RRC ID 64658
著者 Imashiro C, Kurashina Y, Kuribara T, Hirano M, Totani K, Takemura K.
タイトル Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated From Resonance Vibration of a Disk-Shaped Ultrasonic Transducer.
ジャーナル IEEE Trans Biomed Eng
Abstract Cell patterning methods have been previously reported for cell culture. However, these methods use inclusions or devices that are not used in general cell culture and that might affect cell functionality. Here, we report a cell patterning method that can be conducted on a general cell culture dish without any inclusions by employing a resonance vibration of a disk-shaped ultrasonic transducer located under the dish. A resonance vibration with a single nodal circle patterned C2C12 myoblasts into a circular shape on the dish with 10-min exposure of the vibration with maximum peak-peak amplitude of 10 μm[Formula: see text]. Furthermore, the relationship between the amplitude distribution of the transducer and the cell density in the patterned sample could be expressed as a linear function, and there was a clear threshold of amplitude for cell adhesion. To evaluate the cell function of the patterned cells, we conducted proliferation and protein assays at 120-h culture after patterning. Our results showed that the cell proliferation rate did not decrease and the expression of cellular proteins was unchanged. Thus, we conclude, this method can successfully pattern cells in the clinically ubiquitous culture dish, while maintaining cell functionality.
巻・号 66(1)
ページ 111-118
公開日 2019-1-1
DOI 10.1109/TBME.2018.2835834
PMID 29993416
MeSH Animals Bioengineering* / instrumentation Bioengineering* / methods Cell Adhesion / physiology Cell Culture Techniques / instrumentation* Cell Culture Techniques / methods Cell Line Equipment Design Mice Pressure Transducers Ultrasonics / instrumentation*
IF 4.424
リソース情報
ヒト・動物細胞 C2C12(RCB0987)