Reference - Detail
| RRC ID | 64664 |
|---|---|
| Author | Vizcardo R, Rafiqul Islam SM, Maeda T, Tamaoki N, Good ML, Klemen ND, Bosch-Marce M, Jia L, Kruhlak MJ, Restifo NP. |
| Title | A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants. |
| Journal | J Vis Exp |
| Abstract |
The inheritance of pre-rearranged T cell receptors (TCRs) and their epigenetic rejuvenation make induced pluripotent stem cell (iPSC)-derived T cells a promising source for adoptive T cell therapy (ACT). However, classical in vitro methods for producing regenerated T cells from iPSC result in either innate-like or terminally differentiated T cells, which are phenotypically and functionally distinct from naïve T cells. Recently, a novel three-dimensional (3D) thymic culture system was developed to generate a homogenous subset of CD8αβ+ antigen-specific T cells with a naïve T cell-like functional phenotype, including the capacity for proliferation, memory formation, and tumor suppression in vivo. This protocol avoids aberrant developmental fates, allowing for the generation of clinically relevant iPSC-derived T cells, designated as iPSC-derived thymic emigrants (iTE), while also providing a potent tool to elucidate the subsequent functions necessary for T cell maturation after thymic selection. |
| Volume | (150) |
| Published | 2019-8-9 |
| DOI | 10.3791/58672 |
| PMID | 31449236 |
| PMC | PMC8191389 |
| MeSH | Animals Antigens, Neoplasm / immunology* Cell Culture Techniques / methods* Cell Differentiation Cell Line, Tumor Induced Pluripotent Stem Cells / cytology* Mice T-Lymphocytes / cytology T-Lymphocytes / immunology Thymus Gland / cytology* Thymus Gland / immunology* |
| IF | 1.163 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | OP9/N-DLL1(RCB2927) |