| RRC ID |
64679
|
| Author |
Haque M, Xiong X, Lei F, Das JK, Song J.
|
| Title |
An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells.
|
| Journal |
STAR Protoc
|
| Abstract |
In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).
|
| Volume |
2(1)
|
| Pages |
100264
|
| Published |
2021-3-19
|
| DOI |
10.1016/j.xpro.2020.100264
|
| PII |
S2666-1667(20)30251-3
|
| PMID |
33490980
|
| PMC |
PMC7806515
|
| MeSH |
Animals
Hepatitis B / immunology
Hepatitis B / therapy
Hepatitis B Antigens / immunology*
Hepatitis B virus / immunology*
Humans
Immunotherapy, Adoptive
Induced Pluripotent Stem Cells / immunology*
Mice
T-Lymphocytes, Cytotoxic / immunology*
|
| Resource |
| Human and Animal Cells |
iPS-MEF-Ng-20D-17(APS0001) |
