| RRC ID |
64683
|
| Author |
Suzuki H, Kasai K, Kimura Y, Miyata S.
|
| Title |
UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture.
|
| Journal |
Mater Sci Eng C Mater Biol Appl
|
| Abstract |
Culturing pluripotent stem cells effectively requires substrates coated with feeder cell layers or cell-adhesive matrices. It is difficult to employ pluripotent stem cells as resources for regenerative medicine due to risks of culture system contamination by animal-derived factors, or the large costs associated with the use of adhesive matrices. To enable a coating-free culture system, we focused on UV/ozone surface modification and atmospheric pressure plasma treatment for polystyrene substrates, to improve adhesion and proliferation of pluripotent stem cells. In this study, to develop a feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on polystyrene substrates that were surface-modified using UV/ozone-plasma combined treatment. mESCs could be successfully cultured under feeder-free conditions upon UV/ozone-plasma combined treatment of culture substrates, without any further chemical treatments, and showed similar proliferation rates to those of cells grown on the feeder cell layer or matrix-coated substrates.
|
| Volume |
123
|
| Pages |
112012
|
| Published |
2021-4-1
|
| DOI |
10.1016/j.msec.2021.112012
|
| PII |
S0928-4931(21)00151-X
|
| PMID |
33812631
|
| MeSH |
Animals
Atmospheric Pressure
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Feeder Cells
Mice
Ozone*
Plastics
Pluripotent Stem Cells*
|
| IF |
5.88
|
| Resource |
| Human and Animal Cells |
EB3(AES0139) |
