Reference - Detail
| RRC ID | 6475 |
|---|---|
| Author | Oikawa T, Kawai K, Ishiwata I, Ohno T, Akaza H. |
| Title | Induction of potent antitumour natural-killer cells from peripheral blood of patients with advanced prostate cancer. |
| Journal | BJU Int |
| Abstract |
OBJECTIVE:To examine whether antitumour natural-killer (NK) cells can be induced from peripheral blood mononuclear cells (PBMCs) of patients with advanced prostate cancer, as cell therapy using antitumour immune cells is a promising candidate treatment but such patients generally have a suppressed immune response against cancer cells. PATIENTS AND METHODS:PBMCs were obtained from 10 patients (four with stage D2 and six with stage B or C disease). For the NK cell expansion, PBMCs were co-cultured with irradiated HFWT cells, a cell line originating from Wilms' tumour, in RHAM alpha culture medium supplemented with 5% autologous plasma and interleukin-2 (200 U/mL) for 2 weeks. RESULTS:When PBMCs were co-cultured with HFWT cells, lymphocytes from all patients had a 20- to 130-fold expansion after 2 weeks of culture. The CD16+ CD56+ cells constituted >70% of the proliferated lymphocyte population. The induced NK cells had significantly greater cytotoxicity against a prostate cancer cell line (PC-3) than lymphocytes cultured with no HFWT cells. There was no significant difference in growth and phenotypes of lymphocytes and the induced NK cell activity between patients with stage D2, B or C. CONCLUSION:NK cells with potent cytotoxic activity against prostate cancer cell lines from patients with advanced prostate cancer were selectively expanded. Further investigation is needed to determine whether this approach could be a candidate for cell therapy for advanced prostate cancer. |
| Volume | 92(9) |
| Pages | 1009-15 |
| Published | 2003-12-1 |
| DOI | 10.1111/j.1464-410x.2003.04509.x |
| PII | 4509 |
| PMID | 14632866 |
| MeSH | Aged Aged, 80 and over Cell Line, Tumor Cell- and Tissue-Based Therapy / methods Cytotoxicity, Immunologic Histocompatibility Antigens Class I / analysis Humans Immunity, Cellular Immunotherapy / methods* Killer Cells, Natural / immunology* Leukocytes, Mononuclear / immunology* Lymphocyte Activation Male Middle Aged Prostatic Neoplasms / therapy* |
| IF | 4.097 |
| Times Cited | 125 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | HFWT(RCB0665) |