RRC ID 6476
Author Mizuarai S, Ono K, You J, Kamihira M, Iijima S.
Title Protamine-modified DDAB lipid vesicles promote gene transfer in the presence of serum.
Journal J Biochem
Abstract Cationic lipid vesicle-mediated gene transfer has become common for in vitro gene delivery. However, the transfection efficiency is often impaired by serum. DDAB (dimethyldioctadecyl ammonium bromide) lipid vesicle-mediated gene transfer, which we previously reported, has the same problem. To overcome this obstacle, we here report a novel transfection vehicle using protamine-modified DDAB lipid vesicles. While free protamine was simply added to the DNA/lipid complex in the previous study, in the present method the protamine is chemically conjugated to stearic acid and incorporated into DDAB lipid vesicles. Gene transfer was not significantly inhibited in 10% serum-containing medium by this method for the transfection of cultured cells. Protamine-modified DDAB lipid vesicles also enhanced virus transduction efficiency in the presence of serum using a replication-defective retroviral vector. Furthermore, the vesicles allowed efficient gene transfer for avian embryos in vivo. These results indicate that the method is useful for the production of transgenic animals and gene therapy.
Volume 129(1)
Pages 125-32
Published 2001-1-1
DOI 10.1093/oxfordjournals.jbchem.a002822
PMID 11134966
MeSH 3T3 Cells Animals CHO Cells COS Cells Cricetinae DNA / chemistry DNA / genetics* Embryo, Mammalian / physiology Embryo, Nonmammalian Gene Transfer Techniques* Mice Protamines / chemistry* Quail Quaternary Ammonium Compounds / chemistry* Retroviridae / genetics Transfection / methods
IF 2.23
Times Cited 23
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells COS-7(RCB0539) NIH3T3 CHO-K1