論文 - 詳細
| RRC ID | 6478 |
|---|---|
| 著者 | Sakamoto T, Sakata SF, Matsuda K, Horikawa Y, Tamaki N. |
| タイトル | Expression and properties of human liver beta-ureidopropionase. |
| ジャーナル | J Nutr Sci Vitaminol (Tokyo) |
| Abstract |
A cDNA encoding beta-ureidopropionase (BUP) was isolated from a human liver cDNA library, expressed in E. coli, and purified from the culture extract. The 2,006 bp cDNA contained a 1,152 bp open reading frame encoding a protein of 384 amino acids with a molecular weight of 43,165 Da. The subunit molecular weight of the enzyme expressed was about 43,000 Da. The enzyme was inhibited by 1 mM propionate, but not by 10 mM beta-alanine. Chemical analysis of the purified human BUP showed 0.54 zinc atoms per subunit, and the sequence of BUP cDNA contained one putative zinc-binding site motif. The purified enzyme had a pI of 5.65, and exhibited positive cooperativity with N-carbamoyl-beta-alanine as the substrate with a Hill coefficient 2.0. These properties of human BUP, except the inhibition by beta-alanine, were similar to the rat liver purified enzyme. Beta-alanine inhibits rats BUP activity. The complex regulatory function and the negative cooperative mechanism of BUP by beta-alanine have been observed in rats. This kind of mechanism may not exist in humans, because beta-alanine did not inhibit human BUP. |
| 巻・号 | 47(2) |
| ページ | 132-8 |
| 公開日 | 2001-4-1 |
| DOI | 10.3177/jnsv.47.132 |
| PMID | 11508704 |
| MeSH | Amidohydrolases / chemistry Amidohydrolases / genetics* Amidohydrolases / metabolism Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary / chemistry DNA, Complementary / isolation & purification Gene Expression Regulation, Enzymologic Humans Liver / enzymology* Molecular Sequence Data Molecular Weight Open Reading Frames Rats Sequence Alignment Tissue Distribution |
| IF | 1.424 |
| 引用数 | 16 |
| WOS 分野 | NUTRITION & DIETETICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U-937 DE-4(RCB0435) |