| RRC ID |
64787
|
| Author |
Kuriki M, Sato F, Arai HN, Sogabe M, Kaneko M, Kiyonari H, Kawakami K, Yoshimoto Y, Shukunami C, Sehara-Fujisawa A.
|
| Title |
Transient and lineage-restricted requirement of Ebf3 for sternum ossification.
|
| Journal |
Development
|
| Abstract |
Osteoblasts arise from bone-surrounding connective tissue containing tenocytes and fibroblasts. Lineages of these cell populations and mechanisms of their differentiation are not well understood. Screening enhancer-trap lines of zebrafish allowed us to identify Ebf3 as a transcription factor marking tenocytes and connective tissue cells in skeletal muscle of embryos. Knockout of Ebf3 in mice had no effect on chondrogenesis but led to sternum ossification defects as a result of defective generation of Runx2+ pre-osteoblasts. Conditional and temporal Ebf3 knockout mice revealed requirements of Ebf3 in the lateral plate mesenchyme cells (LPMs), especially in tendon/muscle connective tissue cells, and a stage-specific Ebf3 requirement at embryonic day 9.5-10.5. Upregulated expression of connective tissue markers, such as Egr1/2 and Osr1, increased number of Islet1+ mesenchyme cells, and downregulation of gene expression of the Runx2 regulator Shox2 in Ebf3-deleted thoracic LPMs suggest crucial roles of Ebf3 in the onset of lateral plate mesoderm differentiation towards osteoblasts forming sternum tissues.
|
| Volume |
147(9)
|
| Published |
2020-5-12
|
| DOI |
10.1242/dev.186239
|
| PII |
147/9/dev186239
|
| PMID |
32398354
|
| IF |
5.611
|
| Resource |
| Zebrafish |
SAGFF(LF)19A
Tg(UAS:GFP) |
