論文 - 詳細
| RRC ID | 64809 |
|---|---|
| 著者 | Jang YR, Cho K, Kim SW, Altenbach SB, Lim SH, Sim JR, Lee JY. |
| タイトル | Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat. |
| ジャーナル | Molecules |
| Abstract |
Because high-molecular-weight glutenin subunits (HMW-GS) are important contributors to wheat end-use quality, there is a need for high-throughput identification of HMW-GS in wheat genetic resources and breeding lines. We developed an optimized method using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to distinguish individual HMW-GS by considering the effects of the alkylating reagent in protein extraction, solvent components, dissolving volume, and matrix II components. Using the optimized method, 18 of 22 HMW-GS were successfully identified in standard wheat cultivars by differences in molecular weights or by their associations with other tightly linked subunits. Interestingly, 1Bx7 subunits were divided into 1Bx7 group 1 and 1Bx7 group 2 proteins with molecular weights of about 82,400 and 83,000 Da, respectively. Cultivars containing the 1Bx7 group 2 proteins were distinguished from those containing 1Bx7OE using well-known DNA markers. HMW-GS 1Ax2* and 1Bx6 and 1By8 and 1By8*, which are difficult to distinguish due to very similar molecular weights, were easily identified using RP-HPLC. To validate the method, HMW-GS from 38 Korean wheat varieties previously evaluated by SDS-PAGE combined with RP-HPLC were analyzed by MALDI-TOF-MS. The optimized MALDI-TOF-MS method will be a rapid, high-throughput tool for selecting lines containing desirable HMW-GS for breeding efforts. |
| 巻・号 | 25(18) |
| 公開日 | 2020-9-22 |
| DOI | 10.3390/molecules25184347 |
| PII | molecules25184347 |
| PMID | 32971947 |
| PMC | PMC7571055 |
| MeSH | Glutens / analysis* Glutens / chemistry* Molecular Weight Protein Subunits / analysis* Protein Subunits / chemistry* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Triticum / chemistry* |
| IF | 3.267 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| コムギ | Chinese Spring |