| RRC ID |
64948
|
| 著者 |
Yamamoto K, Mohri S, Furuya S.
|
| タイトル |
D-3-phosphoglycerate dehydrogenase from the silkworm Bombyx mori: Identification, functional characterization, and expression.
|
| ジャーナル |
Arch Insect Biochem Physiol
|
| Abstract |
D-3-phosphoglycerate dehydrogenase (PHGDH) is a key enzyme involved in the synthesis of l-serine. Despite the high serine content in silk proteins and the crucial role of PHGDH in serine biosynthesis, PHGDH has not been described in silkworms to date. Here, we identified PHGDH in the silkworm Bombyx mori and evaluated its biochemical properties. On the basis of the amino acid sequence and phylogenetic tree, this PHGDH has been categorized as a new type and designated as bmPHGDH. The recombinant bmPHGDH was overexpressed and purified to homogeneity. Kinetic studies revealed that PHGDH uses NADH as a coenzyme to reduce phosphohydroxypyruvate. High expression levels of bmphgdh messenger RNA (mRNA) were observed in the middle part of the silk gland and midgut in a standard strain of silkworm. Moreover, a sericin-deficient silkworm strain displayed reduced expression of bmphgdh mRNA. These findings indicate that bmPHGDH might play a crucial role in the provision of l-serine in the larva of B. mori.
|
| 巻・号 |
106(1)
|
| ページ |
e21751
|
| 公開日 |
2021-1-1
|
| DOI |
10.1002/arch.21751
|
| PMID |
33058282
|
| MeSH |
Animals
Bombyx* / genetics
Bombyx* / metabolism
Gene Expression
Genes, Insect
Insect Proteins / metabolism
Larva / metabolism
Phosphoglycerate Dehydrogenase* / analysis
Phosphoglycerate Dehydrogenase* / genetics
Phosphoglycerate Dehydrogenase* / metabolism
Phylogeny
Serine / biosynthesis*
|
| IF |
1.536
|
| リソース情報 |
| カイコ |
|
