Reference - Detail
| RRC ID | 64955 |
|---|---|
| Author | Fujita R, Hino M, Ebihara T, Nagasato T, Masuda A, Lee JM, Fujii T, Mon H, Kakino K, Nagai R, Tanaka M, Tonooka Y, Moriyama T, Kusakabe T. |
| Title | Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
In the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines. |
| Volume | 529(2) |
| Pages | 257-262 |
| Published | 2020-8-20 |
| DOI | 10.1016/j.bbrc.2020.06.020 |
| PII | S0006-291X(20)31214-6 |
| PMID | 32703420 |
| PMC | PMC7280120 |
| MeSH | Animals Bombyx / cytology* Bombyx / enzymology Bombyx / virology* Cell Line Cloning, Molecular Furin / metabolism Nucleopolyhedroviruses / genetics* Nucleopolyhedroviruses / metabolism Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Spike Glycoprotein, Coronavirus / biosynthesis* Spike Glycoprotein, Coronavirus / chemistry Spike Glycoprotein, Coronavirus / genetics Spike Glycoprotein, Coronavirus / isolation & purification* |
| IF | 2.985 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | d17 f38 |