| RRC ID |
65009
|
| 著者 |
Gómez-Saldivar G, Fernandez A, Hirano Y, Mauro M, Lai A, Ayuso C, Haraguchi T, Hiraoka Y, Piano F, Askjaer P.
|
| タイトル |
Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Nucleoporins are the constituents of nuclear pore complexes (NPCs) and are essential regulators of nucleocytoplasmic transport, gene expression and genome stability. The nucleoporin MEL-28/ELYS plays a critical role in post-mitotic NPC reassembly through recruitment of the NUP107-160 subcomplex, and is required for correct segregation of mitotic chromosomes. Here we present a systematic functional and structural analysis of MEL-28 in C. elegans early development and human ELYS in cultured cells. We have identified functional domains responsible for nuclear envelope and kinetochore localization, chromatin binding, mitotic spindle matrix association and chromosome segregation. Surprisingly, we found that perturbations to MEL-28's conserved AT-hook domain do not affect MEL-28 localization although they disrupt MEL-28 function and delay cell cycle progression in a DNA damage checkpoint-dependent manner. Our analyses also uncover a novel meiotic role of MEL-28. Together, these results show that MEL-28 has conserved structural domains that are essential for its fundamental roles in NPC assembly and chromosome segregation.
|
| 巻・号 |
12(6)
|
| ページ |
e1006131
|
| 公開日 |
2016-6-1
|
| DOI |
10.1371/journal.pgen.1006131
|
| PII |
PGENETICS-D-16-00581
|
| PMID |
27341616
|
| PMC |
PMC4920428
|
| MeSH |
Active Transport, Cell Nucleus / genetics
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics*
Cell Cycle / genetics
Cell Line, Tumor
Chromatin / genetics
Chromosome Segregation / genetics*
DNA-Binding Proteins / genetics*
HeLa Cells
Humans
K562 Cells
Nuclear Envelope / genetics
Nuclear Pore / genetics
Nuclear Pore Complex Proteins / genetics*
Nuclear Proteins / genetics*
Spindle Apparatus / genetics
|
| IF |
5.175
|
| リソース情報 |
| ヒト・動物細胞 |
K562 |
