RRC ID 65033
著者 Yu YC, Sohma Y, Takimoto S, Miyauchi T, Yasui M.
タイトル Direct visualization and quantitative analysis of water diffusion in complex biological tissues using CARS microscopy.
ジャーナル Sci Rep
Abstract To date, it has not been possible to measure microscopic diffusive water movements in epithelia and in the interstitial space of complex tissues and organs. Diffusive water movements are essential for life because they convey physiologically important small molecules, e.g. nutrients and signaling ligands throughout the extracellular space of complex tissues. Here we report the development of a novel method for the direct observation and quantitative analysis of water diffusion dynamics in a biologically organized tissue using Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Using a computer simulation model to analyze the CARS O-H bond vibration data during H2O/D2O exchange in a 3D epithelial cyst, we succeeded in measuring the diffusive water permeability of the individual luminal and basolateral water pathways and also their response to hormonal stimulation. Our technique will be applicable to the measurement of diffusive water movements in other structurally complex and medically important tissues and organs.
巻・号 3
ページ 2745
公開日 2013-9-25
DOI 10.1038/srep02745
PII srep02745
PMID 24067894
PMC PMC3783033
MeSH Animals Aquaporin 4 / metabolism Cell Membrane Permeability Deuterium Exchange Measurement Diffusion Dogs Epithelial Cells / metabolism Epithelium / drug effects Epithelium / metabolism* Madin Darby Canine Kidney Cells Mice Microscopy / methods* Models, Biological Reproducibility of Results Signal Processing, Computer-Assisted Spectrum Analysis, Raman* Transfection Vasopressins / pharmacology Water / metabolism*
IF 3.998
リソース情報
ヒト・動物細胞 MDCK(RCB0995) M1(RCB0169)