| RRC ID |
65045
|
| 著者 |
Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, Matsui H, Kimura A, Sawa H, Nakayama K, Shibuya H, Nagashima K, Takahashi T.
|
| タイトル |
Cloning and characterization of a rat ortholog of MMP-23 (matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development.
|
| ジャーナル |
Mol Endocrinol
|
| Abstract |
In our attempt to study the role of matrix metalloproteinases (MMPs) in the process of mammalian ovulation, we isolated a rat ortholog of the recently reported human MMP-23 from gonadotropin-primed immature rat ovaries. Transient expression of epitope-tagged rat and human MMP-23 in COS-1 cells revealed that they were synthesized as a membrane-anchored glycoprotein with type II topology. Indirect immunofluorescent analysis showed that subcellular localization of MMP-23 was predominantly in the perinuclear regions. The transfected human MMP-23 protein was processed endogenously to the soluble form in COS-1 cells. However, cotransfection of MMP-23 with the mouse furin cDNA did not enhance this processing, indicating that furin may not be involved in this event. Notably, in situ hybridization analysis revealed a dramatic switching of MMP-23 mRNA localization from granulosa cells to theca-externa/fibroblasts and ovarian surface epithelium during the follicular development. In serum-free primary culture of rat granulosa cells, a drastic diminution of MMP-23 mRNA expression was observed in response to FSH action between 24 h and 48 h of culture. The observed effect of FSH on MMP-23 expression was mimicked by treatment of granulosa cells with forskolin or 8-bromo (Br)-cAMP. In contrast, MMP-23 mRNA levels increased in theca-interstitial cells regardless of the presence of LH in the culture. However, treatment of theca-interstitial cells with forskolin or 8-Br-cAMP markedly reduced the expression of MMP-23 with a concomitant increase in progesterone production. These results indicate that the MMP-23 gene is spatially and temporally regulated in a cell type-specific manner in ovary via the cAMP signaling pathway.
|
| 巻・号 |
15(5)
|
| ページ |
747-64
|
| 公開日 |
2001-5-1
|
| DOI |
10.1210/mend.15.5.0638
|
| PMID |
11328856
|
| MeSH |
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Colforsin / pharmacology
Female
Follicle Stimulating Hormone / physiology
Furin
Gene Expression Regulation, Developmental*
Granulosa Cells / enzymology
Humans
Immunohistochemistry
Luteinizing Hormone / physiology
Matrix Metalloproteinases
Metalloendopeptidases / biosynthesis
Metalloendopeptidases / genetics*
Metalloendopeptidases / physiology
Molecular Sequence Data
Ovarian Follicle / enzymology
Ovarian Follicle / physiology*
Ovary / metabolism
Rats
Rats, Sprague-Dawley
Sequence Homology, Amino Acid
Signal Transduction
Subtilisins / pharmacology
Theca Cells / enzymology
|
| IF |
3.628
|
| リソース情報 |
| ヒト・動物細胞 |
COS-1(RCB0143) |
