RRC ID 65045
Author Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, Matsui H, Kimura A, Sawa H, Nakayama K, Shibuya H, Nagashima K, Takahashi T.
Title Cloning and characterization of a rat ortholog of MMP-23 (matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development.
Journal Mol Endocrinol
Abstract In our attempt to study the role of matrix metalloproteinases (MMPs) in the process of mammalian ovulation, we isolated a rat ortholog of the recently reported human MMP-23 from gonadotropin-primed immature rat ovaries. Transient expression of epitope-tagged rat and human MMP-23 in COS-1 cells revealed that they were synthesized as a membrane-anchored glycoprotein with type II topology. Indirect immunofluorescent analysis showed that subcellular localization of MMP-23 was predominantly in the perinuclear regions. The transfected human MMP-23 protein was processed endogenously to the soluble form in COS-1 cells. However, cotransfection of MMP-23 with the mouse furin cDNA did not enhance this processing, indicating that furin may not be involved in this event. Notably, in situ hybridization analysis revealed a dramatic switching of MMP-23 mRNA localization from granulosa cells to theca-externa/fibroblasts and ovarian surface epithelium during the follicular development. In serum-free primary culture of rat granulosa cells, a drastic diminution of MMP-23 mRNA expression was observed in response to FSH action between 24 h and 48 h of culture. The observed effect of FSH on MMP-23 expression was mimicked by treatment of granulosa cells with forskolin or 8-bromo (Br)-cAMP. In contrast, MMP-23 mRNA levels increased in theca-interstitial cells regardless of the presence of LH in the culture. However, treatment of theca-interstitial cells with forskolin or 8-Br-cAMP markedly reduced the expression of MMP-23 with a concomitant increase in progesterone production. These results indicate that the MMP-23 gene is spatially and temporally regulated in a cell type-specific manner in ovary via the cAMP signaling pathway.
Volume 15(5)
Pages 747-64
Published 2001-5-1
DOI 10.1210/mend.15.5.0638
PMID 11328856
MeSH 8-Bromo Cyclic Adenosine Monophosphate / pharmacology Amino Acid Sequence Animals Base Sequence Cloning, Molecular Colforsin / pharmacology Female Follicle Stimulating Hormone / physiology Furin Gene Expression Regulation, Developmental* Granulosa Cells / enzymology Humans Immunohistochemistry Luteinizing Hormone / physiology Matrix Metalloproteinases Metalloendopeptidases / biosynthesis Metalloendopeptidases / genetics* Metalloendopeptidases / physiology Molecular Sequence Data Ovarian Follicle / enzymology Ovarian Follicle / physiology* Ovary / metabolism Rats Rats, Sprague-Dawley Sequence Homology, Amino Acid Signal Transduction Subtilisins / pharmacology Theca Cells / enzymology
IF 3.628
Human and Animal Cells COS-1(RCB0143)