論文 - 詳細
| RRC ID | 65045 |
|---|---|
| 著者 | Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, Matsui H, Kimura A, Sawa H, Nakayama K, Shibuya H, Nagashima K, Takahashi T. |
| タイトル | Cloning and characterization of a rat ortholog of MMP-23 (matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development. |
| ジャーナル | Mol Endocrinol |
| Abstract |
In our attempt to study the role of matrix metalloproteinases (MMPs) in the process of mammalian ovulation, we isolated a rat ortholog of the recently reported human MMP-23 from gonadotropin-primed immature rat ovaries. Transient expression of epitope-tagged rat and human MMP-23 in COS-1 cells revealed that they were synthesized as a membrane-anchored glycoprotein with type II topology. Indirect immunofluorescent analysis showed that subcellular localization of MMP-23 was predominantly in the perinuclear regions. The transfected human MMP-23 protein was processed endogenously to the soluble form in COS-1 cells. However, cotransfection of MMP-23 with the mouse furin cDNA did not enhance this processing, indicating that furin may not be involved in this event. Notably, in situ hybridization analysis revealed a dramatic switching of MMP-23 mRNA localization from granulosa cells to theca-externa/fibroblasts and ovarian surface epithelium during the follicular development. In serum-free primary culture of rat granulosa cells, a drastic diminution of MMP-23 mRNA expression was observed in response to FSH action between 24 h and 48 h of culture. The observed effect of FSH on MMP-23 expression was mimicked by treatment of granulosa cells with forskolin or 8-bromo (Br)-cAMP. In contrast, MMP-23 mRNA levels increased in theca-interstitial cells regardless of the presence of LH in the culture. However, treatment of theca-interstitial cells with forskolin or 8-Br-cAMP markedly reduced the expression of MMP-23 with a concomitant increase in progesterone production. These results indicate that the MMP-23 gene is spatially and temporally regulated in a cell type-specific manner in ovary via the cAMP signaling pathway. |
| 巻・号 | 15(5) |
| ページ | 747-64 |
| 公開日 | 2001-5-1 |
| DOI | 10.1210/mend.15.5.0638 |
| PMID | 11328856 |
| MeSH | 8-Bromo Cyclic Adenosine Monophosphate / pharmacology Amino Acid Sequence Animals Base Sequence Cloning, Molecular Colforsin / pharmacology Female Follicle Stimulating Hormone / physiology Furin Gene Expression Regulation, Developmental* Granulosa Cells / enzymology Humans Immunohistochemistry Luteinizing Hormone / physiology Matrix Metalloproteinases Metalloendopeptidases / biosynthesis Metalloendopeptidases / genetics* Metalloendopeptidases / physiology Molecular Sequence Data Ovarian Follicle / enzymology Ovarian Follicle / physiology* Ovary / metabolism Rats Rats, Sprague-Dawley Sequence Homology, Amino Acid Signal Transduction Subtilisins / pharmacology Theca Cells / enzymology |
| IF | 3.628 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-1(RCB0143) |