| RRC ID |
65079
|
| Author |
Ogawa Y, Noda K, Kimura S, Kitaoka M, Wada M.
|
| Title |
Facile preparation of highly crystalline lamellae of (1 → 3)-β-D-glucan using an extract of Euglena gracilis.
|
| Journal |
Int J Biol Macromol
|
| Abstract |
In vitro synthesis of (1 → 3)-β-D-glucan was performed using laminaribiose phosphorylase obtained by an extraction of Euglena gracilis with sucrose phosphorylase. The synthetic product was a linear (1 → 3)-β-D-glucan with a narrow distribution of degree of polymerization (DP) centered on DP=30. X-ray diffraction and electron microscopy revealed that the glucan molecules obtained were self-organized as highly crystalline hexagonal lamellae. This synthetic product has quite high structural homogeneity at every level from primary to higher-order structure, which is a great advantage for the detailed analyses of physiological functions of (1 → 3)-β-D-glucan.
|
| Volume |
64
|
| Pages |
415-9
|
| Published |
2014-3-1
|
| DOI |
10.1016/j.ijbiomac.2013.12.027
|
| PII |
S0141-8130(13)00685-5
|
| PMID |
24374085
|
| MeSH |
Euglena gracilis / chemistry*
Glucosyltransferases / chemistry*
Glucosyltransferases / isolation & purification
Microscopy, Electron
Proteoglycans
X-Ray Diffraction
beta-Glucans / chemical synthesis*
|
| IF |
5.162
|
| Resource |
| Algae |
NIES-48 |
