| RRC ID |
6508
|
| 著者 |
Yanagiya A, Jia Q, Ohka S, Horie H, Nomoto A.
|
| タイトル |
Blockade of the poliovirus-induced cytopathic effect in neural cells by monoclonal antibody against poliovirus or the human poliovirus receptor.
|
| ジャーナル |
J Virol
|
| Abstract |
The poliovirus (PV)-induced cytopathic effect (CPE) was blocked in neural cells but not in HeLa cells by the addition of monoclonal antibody (MAb) against PV or the human PV receptor (CD155) 2 h postinfection (hpi). Since each MAb has the ability to block viral infection, no CPE in PV-infected neural cells appeared to result from the blockade of multiple rounds of viral replication. Pulse-labeling experiments revealed that virus-specific protein synthesis proceeded 5 hpi with or without MAbs. However, in contrast to the results obtained without MAbs, virus-specific protein synthesis with MAbs was not detected 7 hpi. Shutoff of host translation was also not observed in the presence of MAbs. Western blot analysis showed that 2Apro, the viral protein which mediates the cleavage of eukaryotic translation initiation factor eIF4G, was still present 11 hpi. However, intact eIF4G appeared 11 hpi. An immunocytochemical study indicated that 2Apro was detected only in the nucleus 11 hpi. These results suggest that neural cells possess protective response mechanisms against PV infection as follows: (i) upon PV infection, neural cells produce a factor(s) to suppress PV internal ribosome entry site activity by 7 hpi, (ii) a factor which supports cap-dependent translation for eIF4G may exist in infected cells when no intact eIF4G is detected, and (iii) the remaining 2Apro is not effective in cleaving eIF4G because it is imported into the nucleus by 11 hpi.
|
| 巻・号 |
79(3)
|
| ページ |
1523-32
|
| 公開日 |
2005-2-1
|
| DOI |
10.1128/JVI.79.3.1523-1532.2005
|
| PII |
79/3/1523
|
| PMID |
15650178
|
| PMC |
PMC544096
|
| MeSH |
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology*
Antibodies, Viral / immunology*
Base Sequence
Cell Line, Tumor
Cysteine Endopeptidases / metabolism
Cytopathogenic Effect, Viral / immunology
HeLa Cells
Humans
Membrane Proteins / immunology*
Molecular Sequence Data
Neurons / virology*
Poliovirus / immunology
Poliovirus / pathogenicity*
Receptors, Virus / immunology*
Sequence Analysis, DNA
Viral Proteins / metabolism
Virus Replication
|
| IF |
4.501
|
| 引用数 |
11
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
