RRC ID 6509
著者 Naganuma M, Saruwatari A, Okamura S, Tamura H.
タイトル Turmeric and curcumin modulate the conjugation of 1-naphthol in Caco-2 cells.
ジャーナル Biol Pharm Bull
Abstract Turmeric, the powdered dry rhizome of the Curcuma longa plant, and curcumin, the major anti-oxidant constituent of turmeric, have been shown to possess chemopreventive activity. To elucidate the possible interaction of turmeric and curcumin with conjugation reactions, which in many cases are involved in the activation of procarcinogens, we measured their effects in the conjugation of 1-naphthol in Caco-2 cells, a human colon carcinoma cell line, within a 24 h period. Turmeric exhibits inhibitory activity toward both sulfo- and glucuronosyl conjugations of 1-naphthol at approximately the same levels (IC(50)=0.24 and 0.29 mg/ml, respectively). Curcumin inhibits sulfo-conjugation at lower concentrations (IC(50)=9.7 microg/ml), but only showed weak inhibition toward glucuronosyl conjugation of 1-naphthol in Caco-2 cells. In addition, turmeric was found to strongly inhibit in vitro phenol sulfotransferase (SULT) activity and demonstrate moderate inhibitory properties against UDP-glucuronosyl transferase (UGT) activity in Caco-2 cells (IC(50)=0.17 mg/ml and 0.62 mg/ml, respectively). Curcumin also strongly inhibits in vitro phenol sulfotransferase activity with an IC(50) of 2.4 microg/ml. Moreover, and in contrast to the moderate inhibition of UGT activity by turmeric and curcumin, both induce the expression of the UGT1A1 and UGT1A6 genes, revealed by real-time PCR analysis. These findings are indicative of a possible interaction of both turmeric and curcumin with conjugation reactions in the human intestinal tract and colon. This in turn may affect the bioavailability of therapeutic drugs and toxicity levels of environmental chemicals, particularly procarcinogens.
巻・号 29(7)
ページ 1476-9
公開日 2006-7-1
DOI 10.1248/bpb.29.1476
PII JST.JSTAGE/bpb/29.1476
PMID 16819192
MeSH Adenocarcinoma Cell Line, Tumor Colonic Neoplasms Curcuma* Curcumin / pharmacology* Gene Expression Regulation, Enzymologic / drug effects* Glucuronides / metabolism Humans Kinetics Naphthols / pharmacokinetics*
IF 1.863
引用数 26
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞