RRC ID 65135
Author Jin H, Won M, Shin E, Kim HM, Lee K, Bae J.
Title EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells.
Journal Biochem Biophys Res Commun
Abstract Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene (EGR2) is a gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 promoted survival of KGN human granulosa-derived cells in which IER3 acts as a mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which belongs to the BCL-2 family of proteins regulating cell survival. Thus, this study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, which is important for the survival of granulosa cells during folliculogenesis.
Volume 482(4)
Pages 877-882
Published 2017-1-22
DOI 10.1016/j.bbrc.2016.11.127
PII S0006-291X(16)32001-0
PMID 27890615
MeSH Animals Apoptosis Regulatory Proteins / genetics* Base Sequence Cell Line Cell Survival Early Growth Response Protein 2 / genetics* Early Growth Response Protein 2 / metabolism Female Gonadotropins / metabolism* Granulosa Cells / cytology Granulosa Cells / metabolism* Humans Membrane Proteins / genetics* Promoter Regions, Genetic RNA, Messenger / genetics Rats Rats, Sprague-Dawley Transcriptional Activation*
IF 2.985
Human and Animal Cells KGN(RCB1154)