論文 - 詳細
| RRC ID | 65135 |
|---|---|
| 著者 | Jin H, Won M, Shin E, Kim HM, Lee K, Bae J. |
| タイトル | EGR2 is a gonadotropin-induced survival factor that controls the expression of IER3 in ovarian granulosa cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene (EGR2) is a gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (IER3) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 promoted survival of KGN human granulosa-derived cells in which IER3 acts as a mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which belongs to the BCL-2 family of proteins regulating cell survival. Thus, this study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, which is important for the survival of granulosa cells during folliculogenesis. |
| 巻・号 | 482(4) |
| ページ | 877-882 |
| 公開日 | 2017-1-22 |
| DOI | 10.1016/j.bbrc.2016.11.127 |
| PII | S0006-291X(16)32001-0 |
| PMID | 27890615 |
| MeSH | Animals Apoptosis Regulatory Proteins / genetics* Base Sequence Cell Line Cell Survival Early Growth Response Protein 2 / genetics* Early Growth Response Protein 2 / metabolism Female Gonadotropins / metabolism* Granulosa Cells / cytology Granulosa Cells / metabolism* Humans Membrane Proteins / genetics* Promoter Regions, Genetic RNA, Messenger / genetics Rats Rats, Sprague-Dawley Transcriptional Activation* |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |