| RRC ID |
65147
|
| 著者 |
Muguruma N, Okamoto K, Nakagawa T, Sannomiya K, Fujimoto S, Mitsui Y, Kimura T, Miyamoto H, Higashijima J, Shimada M, Horino Y, Matsumoto S, Hanaoka K, Nagano T, Shibutani M, Takayama T.
|
| タイトル |
Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Aberrant crypt foci (ACF), the earliest precursor lesion of colorectal cancers (CRCs), are a good surrogate marker for CRC risk stratification and chemoprevention. However, the conventional ACF detection method with dye-spraying by magnifying colonoscopy is labor- and skill-intensive. We sought to identify rat and human ACF using a fluorescent imaging technique that targets a molecule specific for ACF. We found that glutathione S-transferase (GST) P1-1 was overexpressed in ACF tissues in a screening experiment. We then synthesized the fluorogenic probe, DNAT-Me, which is fluorescently quenched but is activated by GSTP1-1. A CRC cell line incubated with DNAT-Me showed strong fluorescence in the cytosol. Fluorescence intensities correlated significantly with GST activities in cancer cell lines. When we sprayed DNAT-Me onto colorectal mucosa excised from azoxymethane-treated rats and surgically resected from CRC patients, ACF with strong fluorescent signals were clearly observed. The ACF number determined by postoperative DNAT-Me imaging was almost identical to that determined by preoperative methylene blue staining. The signal-to-noise ratio for ACF in DNAT-Me images was significantly higher than that in methylene blue staining. Thus, we sensitively visualized ACF on rat and human colorectal mucosa by using a GST-activated fluorogenic probe without dye-spraying and magnifying colonoscopy.
|
| 巻・号 |
7(1)
|
| ページ |
6536
|
| 公開日 |
2017-7-26
|
| DOI |
10.1038/s41598-017-06857-x
|
| PII |
10.1038/s41598-017-06857-x
|
| PMID |
28747791
|
| PMC |
PMC5529364
|
| MeSH |
Aberrant Crypt Foci / diagnosis*
Colon / pathology*
Colorectal Neoplasms / diagnosis*
Fluorescent Dyes / analysis
Glutathione Transferase / analysis*
Humans
Molecular Imaging / methods*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
HGC-27(RCB0500)
NUGC-4(RCB1939)
HuH-28(RCB1943) |
