Reference - Detail
| RRC ID | 65149 |
|---|---|
| Author | Tran LS, Tran D, De Paoli A, D'Costa K, Creed SJ, Ng GZ, Le L, Sutton P, Silke J, Nachbur U, Ferrero RL. |
| Title | NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells. |
| Journal | Cell Microbiol |
| Abstract |
Helicobacter pylori (H. pylori) causes chronic inflammation which is a key precursor to gastric carcinogenesis. It has been suggested that H. pylori may limit this immunopathology by inducing the production of interleukin 33 (IL-33) in gastric epithelial cells, thus promoting T helper 2 immune responses. The molecular mechanism underlying IL-33 production in response to H. pylori infection, however, remains unknown. In this study, we demonstrate that H. pylori activates signalling via the pathogen recognition molecule Nucleotide-Binding Oligomerisation Domain-Containing Protein 1 (NOD1) and its adaptor protein receptor-interacting serine-threonine Kinase 2, to promote production of both full-length and processed IL-33 in gastric epithelial cells. Furthermore, IL-33 responses were dependent on the actions of the H. pylori Type IV secretion system, required for activation of the NOD1 pathway, as well as on the Type IV secretion system effector protein, CagA. Importantly, Nod1+/+ mice with chronic H. pylori infection exhibited significantly increased gastric IL-33 and splenic IL-13 responses, but decreased IFN-γ responses, when compared with Nod1-/- animals. Collectively, our data identify NOD1 as an important regulator of mucosal IL-33 responses in H. pylori infection. We suggest that NOD1 may play a role in protection against excessive inflammation. |
| Volume | 20(5) |
| Pages | e12826 |
| Published | 2018-5-1 |
| DOI | 10.1111/cmi.12826 |
| PMID | 29392836 |
| MeSH | Animals Cell Line Epithelial Cells / microbiology Epithelial Cells / pathology Gastric Mucosa / immunology Gastric Mucosa / microbiology Helicobacter Infections / genetics* Helicobacter Infections / immunology Helicobacter Infections / microbiology Helicobacter pylori / genetics Helicobacter pylori / immunology Helicobacter pylori / pathogenicity* Humans Immunity, Mucosal / genetics Inflammation / genetics Inflammation / immunology Inflammation / microbiology Interferon-gamma / genetics Interleukin-33 / genetics* Mice Nod1 Signaling Adaptor Protein / genetics* Receptors, Interleukin-13 / genetics* Th2 Cells / immunology Th2 Cells / microbiology |
| IF | 3.43 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | GSM06(RCB1779) |