論文 - 詳細
| RRC ID | 65158 |
|---|---|
| 著者 | Haque M, Lei F, Xiong X, Ren Y, Peng HY, Wang L, Kumar A, Das JK, Song J. |
| タイトル | Stem Cell-Derived Viral Antigen-Specific T Cells Suppress HIV Replication and PD-1 Expression on CD4+ T Cells. |
| ジャーナル | Viruses |
| Abstract |
The viral antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) derived from pluripotent stem cells (PSCs), i.e., PSC-CTLs, have the ability to suppress the human immunodeficiency virus (HIV) infection. After adoptive transfer, PSC-CTLs can infiltrate into the local tissues to suppress HIV replication. Nevertheless, the mechanisms by which the viral Ag-specific PSC-CTLs elicit the antiviral response remain to be fully elucidated. In this study, we generated the functional HIV-1 Gag epitope SL9-specific CTLs from the induced PSC (iPSCs), i.e., iPSC-CTLs, and investigated the suppression of SL9-specific iPSC-CTLs on viral replication and the protection of CD4+ T cells. A chimeric HIV-1, i.e., EcoHIV, was used to produce HIV replication in mice. We show that adoptive transfer of SL9-specific iPSC-CTLs greatly suppressed EcoHIV replication in the peritoneal macrophages and spleen in the animal model. Furthermore, we demonstrate that the adoptive transfer significantly reduced expression of PD-1 on CD4+ T cells in the spleen and generated persistent anti-HIV memory T cells. These results indicate that stem cell-derived viral Ag-specific CTLs can robustly accumulate in the local tissues to suppress HIV replication and prevent CD4+ T cell exhaustion through reduction of PD-1 expression. |
| 巻・号 | 13(5) |
| 公開日 | 2021-4-25 |
| DOI | 10.3390/v13050753 |
| PII | v13050753 |
| PMID | 33923025 |
| PMC | PMC8146941 |
| MeSH | Adoptive Transfer Animals Antigens, Viral / immunology* Antigens, Viral / metabolism CD4-Positive T-Lymphocytes / immunology Epitopes, T-Lymphocyte / immunology HIV / genetics* HIV / immunology* HIV / physiology HIV Infections / immunology Humans Induced Pluripotent Stem Cells Memory T Cells / immunology Mice Mice, Transgenic Programmed Cell Death 1 Receptor / genetics* Programmed Cell Death 1 Receptor / immunology T-Lymphocytes, Cytotoxic / immunology T-Lymphocytes, Cytotoxic / virology* Virus Replication / genetics* Virus Replication / immunology* gag Gene Products, Human Immunodeficiency Virus / genetics gag Gene Products, Human Immunodeficiency Virus / immunology |
| IF | 3.816 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |