論文 - 詳細
| RRC ID | 65223 |
|---|---|
| 著者 | Heissenberger C, Rollins JA, Krammer TL, Nagelreiter F, Stocker I, Wacheul L, Shpylovyi A, Tav K, Snow S, Grillari J, Rogers AN, Lafontaine DLJ, Schosserer M. |
| タイトル | The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans. |
| ジャーナル | Elife |
| Abstract |
Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second known 26S rRNA m5C at position C2982. Depletion of nsun-1 or nsun-5 improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, soma-specific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 depletion, translation of specific mRNAs was remodeled leading to reduced production of collagens, loss of structural integrity of the cuticle, and impaired barrier function. We conclude that loss of a single enzyme required for rRNA methylation has profound and highly specific effects on organismal development and physiology. |
| 巻・号 | 9 |
| 公開日 | 2020-12-8 |
| DOI | 10.7554/eLife.56205 |
| PII | 56205 |
| PMID | 33289480 |
| PMC | PMC7746234 |
| MeSH | Aging / metabolism* Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Female Fertility / physiology Longevity / physiology* Methyltransferases / metabolism* Oogenesis / physiology RNA Processing, Post-Transcriptional / physiology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6081 tm3898 |