RRC ID |
65223
|
Author |
Heissenberger C, Rollins JA, Krammer TL, Nagelreiter F, Stocker I, Wacheul L, Shpylovyi A, Tav K, Snow S, Grillari J, Rogers AN, Lafontaine DLJ, Schosserer M.
|
Title |
The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans.
|
Journal |
Elife
|
Abstract |
Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second known 26S rRNA m5C at position C2982. Depletion of nsun-1 or nsun-5 improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, soma-specific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 depletion, translation of specific mRNAs was remodeled leading to reduced production of collagens, loss of structural integrity of the cuticle, and impaired barrier function. We conclude that loss of a single enzyme required for rRNA methylation has profound and highly specific effects on organismal development and physiology.
|
Volume |
9
|
Published |
2020-12-8
|
DOI |
10.7554/eLife.56205
|
PII |
56205
|
PMID |
33289480
|
PMC |
PMC7746234
|
MeSH |
Aging / metabolism*
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins / metabolism*
Female
Fertility / physiology
Longevity / physiology*
Methyltransferases / metabolism*
Oogenesis / physiology
RNA Processing, Post-Transcriptional / physiology
|
Resource |
C.elegans |
tm6081
tm3898 |