| RRC ID |
6525
|
| Author |
Minamizato T, Sakamoto K, Liu T, Kokubo H, Katsube K, Perbal B, Nakamura S, Yamaguchi A.
|
| Title |
CCN3/NOV inhibits BMP-2-induced osteoblast differentiation by interacting with BMP and Notch signaling pathways.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We elucidate the role of CCN3/NOV, a member of the CCN family proteins, in osteoblast differentiation using MC3T3-E1 osteoblastic cells. Transduction with CCN3 adenovirus (AdCCN3) alone induced no apparent changes in the expression of osteoblast-related markers, whereas cotransduction with BMP-2 adenovirus (AdBMP-2) and AdCCN3 significantly inhibited the AdBMP-2-induced mRNA expression of Runx2, osterix, ALP, and osteocalcin. Immunoprecipitation-western analysis revealed that CCN3 associated with BMP-2. Compared to transduction with AdBMP-2 alone, cotransduction with AdBMP-2 and AdCCN3 attenuated the expression of phosphorylated Smad1/5/8 and the mRNA for Id1, Id2, and Id3. Transduction with AdCCN3 stimulated the expression of cleaved Notch1, the mRNA expression of Hes1 and Hey1/Hesr1, and the promoter activities of Hes1 and Hey1. The inhibitory effects of CCN3 on the expression of BMP-2-induced osteoblast-related markers were nullified in Hey1-deficient osteoblastic cells. These results indicate that CCN3 exerts inhibitory effects on BMP-2-induced osteoblast differentiation by its involvement of the BMP and Notch signaling pathways.
|
| Volume |
354(2)
|
| Pages |
567-73
|
| Published |
2007-3-9
|
| DOI |
10.1016/j.bbrc.2007.01.029
|
| PII |
S0006-291X(07)00049-6
|
| PMID |
17250806
|
| MeSH |
3T3 Cells
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / antagonists & inhibitors*
Cell Differentiation / physiology*
Connective Tissue Growth Factor
Humans
Immediate-Early Proteins / physiology*
Intercellular Signaling Peptides and Proteins / physiology*
Mice
Nephroblastoma Overexpressed Protein
Osteoblasts / physiology*
Receptors, Notch / physiology*
Signal Transduction / physiology*
Transforming Growth Factor beta / antagonists & inhibitors*
|
| IF |
2.985
|
| Times Cited |
74
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987)
293(RCB1637) |
