| RRC ID |
65325
|
| 著者 |
Cohen JD, Sparacio AP, Belfi AC, Forman-Rubinsky R, Hall DH, Maul-Newby H, Frand AR, Sundaram MV.
|
| タイトル |
A multi-layered and dynamic apical extracellular matrix shapes the vulva lumen in Caenorhabditis elegans.
|
| ジャーナル |
Elife
|
| Abstract |
Biological tubes must develop and maintain their proper diameter to transport materials efficiently. These tubes are molded and protected in part by apical extracellular matrices (aECMs) that line their lumens. Despite their importance, aECMs are difficult to image in vivo and therefore poorly understood. The Caenorhabditis elegans vulva has been a paradigm for understanding many aspects of organogenesis. Here we describe the vulva luminal matrix, which contains chondroitin proteoglycans, Zona Pellucida (ZP) domain proteins, and other glycoproteins and lipid transporters related to those in mammals. Confocal and transmission electron microscopy revealed, with unprecedented detail, a complex and dynamic aECM. Different matrix factors assemble on the apical surfaces of each vulva cell type, with clear distinctions seen between Ras-dependent (1°) and Notch-dependent (2°) cell types. Genetic perturbations suggest that chondroitin and other aECM factors together generate a structured scaffold that both expands and constricts lumen shape.
|
| 巻・号 |
9
|
| 公開日 |
2020-9-25
|
| DOI |
10.7554/eLife.57874
|
| PII |
57874
|
| PMID |
32975517
|
| PMC |
PMC7544507
|
| MeSH |
Animals
Caenorhabditis elegans / embryology*
Embryo, Nonmammalian / embryology
Extracellular Matrix / metabolism*
Female
Glycoproteins / metabolism*
Organogenesis*
Vulva / embryology
|
| リソース情報 |
| 線虫 |
tm290 |
