RRC ID 65351
Author Qi W, Gromoff EDV, Xu F, Zhao Q, Yang W, Pfeifer D, Maier W, Long L, Baumeister R.
Title The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans.
Journal Nat Commun
Abstract Multicellular organisms coordinate tissue specific responses to environmental information via both cell-autonomous and non-autonomous mechanisms. In addition to secreted ligands, recent reports implicated release of small RNAs in regulating gene expression across tissue boundaries. Here, we show that the conserved poly-U specific endoribonuclease ENDU-2 in C. elegans is secreted from the soma and taken-up by the germline to ensure germline immortality at elevated temperature. ENDU-2 binds to mature mRNAs and negatively regulates mRNA abundance both in the soma and the germline. While ENDU-2 promotes RNA decay in the soma directly via its endoribonuclease activity, ENDU-2 prevents misexpression of soma-specific genes in the germline and preserves germline immortality independent of its RNA-cleavage activity. In summary, our results suggest that the secreted RNase ENDU-2 regulates gene expression across tissue boundaries in response to temperature alterations and contributes to maintenance of stem cell immortality, probably via retaining a stem cell specific program of gene expression.
Volume 12(1)
Pages 1262
Published 2021-2-24
DOI 10.1038/s41467-021-21516-6
PII 10.1038/s41467-021-21516-6
PMID 33627668
PMC PMC7904951
MeSH Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endoribonucleases / genetics Endoribonucleases / metabolism* Germ-Line Mutation / genetics RNA Stability / genetics RNA Stability / physiology RNA, Messenger / metabolism Stem Cells / cytology Stem Cells / metabolism Temperature
Resource
C.elegans tm4977 tm1200 tm1977