| RRC ID |
65351
|
| Author |
Qi W, Gromoff EDV, Xu F, Zhao Q, Yang W, Pfeifer D, Maier W, Long L, Baumeister R.
|
| Title |
The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans.
|
| Journal |
Nat Commun
|
| Abstract |
Multicellular organisms coordinate tissue specific responses to environmental information via both cell-autonomous and non-autonomous mechanisms. In addition to secreted ligands, recent reports implicated release of small RNAs in regulating gene expression across tissue boundaries. Here, we show that the conserved poly-U specific endoribonuclease ENDU-2 in C. elegans is secreted from the soma and taken-up by the germline to ensure germline immortality at elevated temperature. ENDU-2 binds to mature mRNAs and negatively regulates mRNA abundance both in the soma and the germline. While ENDU-2 promotes RNA decay in the soma directly via its endoribonuclease activity, ENDU-2 prevents misexpression of soma-specific genes in the germline and preserves germline immortality independent of its RNA-cleavage activity. In summary, our results suggest that the secreted RNase ENDU-2 regulates gene expression across tissue boundaries in response to temperature alterations and contributes to maintenance of stem cell immortality, probably via retaining a stem cell specific program of gene expression.
|
| Volume |
12(1)
|
| Pages |
1262
|
| Published |
2021-2-24
|
| DOI |
10.1038/s41467-021-21516-6
|
| PII |
10.1038/s41467-021-21516-6
|
| PMID |
33627668
|
| PMC |
PMC7904951
|
| MeSH |
Animals
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / physiology
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Endoribonucleases / genetics
Endoribonucleases / metabolism*
Germ-Line Mutation / genetics
RNA Stability / genetics
RNA Stability / physiology
RNA, Messenger / metabolism
Stem Cells / cytology
Stem Cells / metabolism
Temperature
|
| Resource |
| C.elegans |
tm4977
tm1200
tm1977 |
