論文 - 詳細
| RRC ID | 6537 |
|---|---|
| 著者 | Miyata S, Fukushima T, Kohama K, Tanaka H, Takeshima H, Kataoka H. |
| タイトル | Roles of Kunitz domains in the anti-invasive effect of hepatocyte growth factor activator inhibitor type 1 in human glioblastoma cells. |
| ジャーナル | Hum Cell |
| Abstract |
Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is a membrane-bound serine proteinase inhibitor having two extracellular Kunitz-type proteinase inhibitor domains (KD) namely KD-1 and KD-2. It efficiently inhibits hepatocyte growth factor activator, matriptase, hepsin, prostasin and trypsin. We have previously reported that the expression of HAI-1 suppresses the in vitro invasive capability of human glioblastoma cells. In this study we examined the role of each KD in the anti-invasive effect of HAI-1. Engineered over-expression of the mature membrane-form HAI-1 suppressed in vitro fibrin gel invasion of two human glioblastoma cell lines, U251 and YKG-1. The migratory activity on type IV collagen was also suppressed by the HAI-1 expression. These effects were not affected by the deletion of intracytoplasmic domain of HAI-1. A truncated secreted form of HAI-1 also suppressed in vitro invasion of the cells, indicating that the extracellular portion of HAI-1 was responsible for the anti-invasive effect. To determine the roles of each KD in the anti-invasive effect of HAI-1 in vitro, we constructed expression plasmids for HAI-1 with or without mutation at the P1 position of the reactive site of each KD. The results revealed that the proteinase inhibitor activity of N-terminal KD (KD-1) is responsible for the anti-invasion effect of HAI-1. |
| 巻・号 | 20(4) |
| ページ | 100-6 |
| 公開日 | 2007-11-1 |
| DOI | 10.1111/j.1749-0774.2007.00035.x |
| PII | HUC035 |
| PMID | 17949349 |
| MeSH | Amino Acid Motifs Glioblastoma / pathology* Humans Neoplasm Invasiveness Protein Structure, Tertiary / physiology Proteinase Inhibitory Proteins, Secretory / chemistry* Proteinase Inhibitory Proteins, Secretory / genetics Proteinase Inhibitory Proteins, Secretory / physiology* Serine Proteinase Inhibitors / physiology* Tumor Cells, Cultured |
| IF | 3.463 |
| 引用数 | 19 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | U251(RCB0461) YKG1(RCB2110) |