論文 - 詳細
| RRC ID | 65382 |
|---|---|
| 著者 | Beltran T, Pahita E, Ghosh S, Lenhard B, Sarkies P. |
| タイトル | Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors. |
| ジャーナル | EMBO J |
| Abstract |
Piwi-interacting RNAs (piRNAs) play key roles in germline development and genome defence in metazoans. In C. elegans, piRNAs are transcribed from > 15,000 discrete genomic loci by RNA polymerase II (Pol II), resulting in 28 nt short-capped piRNA precursors. Here, we investigate transcription termination at piRNA loci. We show that the Integrator complex, which terminates snRNA transcription, is recruited to piRNA loci. Moreover, we demonstrate that the catalytic activity of Integrator cleaves nascent capped piRNA precursors associated with promoter-proximal Pol II, resulting in termination of transcription. Loss of Integrator activity, however, does not result in transcriptional readthrough at the majority of piRNA loci. Taken together, our results draw new parallels between snRNA and piRNA biogenesis in nematodes and provide evidence of a role for the Integrator complex as a terminator of promoter-proximal RNA polymerase II during piRNA biogenesis. |
| 巻・号 | 40(5) |
| ページ | e105564 |
| 公開日 | 2021-3-1 |
| DOI | 10.15252/embj.2020105564 |
| PMID | 33340372 |
| PMC | PMC7917550 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Germ Cells Promoter Regions, Genetic* RNA Caps RNA Polymerase II / genetics RNA Polymerase II / metabolism* RNA Precursors / genetics RNA Precursors / metabolism* RNA Processing, Post-Transcriptional* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Transcription, Genetic |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1616 tm1615 |