論文 - 詳細
| RRC ID | 65383 |
|---|---|
| 著者 | Navarro IC, Tuorto F, Jordan D, Legrand C, Price J, Braukmann F, Hendrick AG, Akay A, Kotter A, Helm M, Lyko F, Miska EA. |
| タイトル | Translational adaptation to heat stress is mediated by RNA 5-methylcytosine in Caenorhabditis elegans. |
| ジャーナル | EMBO J |
| Abstract |
Methylation of carbon-5 of cytosines (m5 C) is a post-transcriptional nucleotide modification of RNA found in all kingdoms of life. While individual m5 C-methyltransferases have been studied, the impact of the global cytosine-5 methylome on development, homeostasis and stress remains unknown. Here, using Caenorhabditis elegans, we generated the first organism devoid of m5 C in RNA, demonstrating that this modification is non-essential. Using this genetic tool, we determine the localisation and enzymatic specificity of m5 C sites in the RNome in vivo. We find that NSUN-4 acts as a dual rRNA and tRNA methyltransferase in C. elegans mitochondria. In agreement with leucine and proline being the most frequently methylated tRNA isoacceptors, loss of m5 C impacts the decoding of some triplets of these two amino acids, leading to reduced translation efficiency. Upon heat stress, m5 C loss leads to ribosome stalling at UUG triplets, the only codon translated by an m5 C34-modified tRNA. This leads to reduced translation efficiency of UUG-rich transcripts and impaired fertility, suggesting a role of m5 C tRNA wobble methylation in the adaptation to higher temperatures. |
| 巻・号 | 40(6) |
| ページ | e105496 |
| 公開日 | 2021-3-15 |
| DOI | 10.15252/embj.2020105496 |
| PMID | 33283887 |
| PMC | PMC7957426 |
| MeSH | 5-Methylcytosine / metabolism* Adaptation, Physiological / genetics* Animals CRISPR-Cas Systems / genetics Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Cytosine / chemistry Gene Editing Heat-Shock Response / genetics* Hot Temperature Leucine / chemistry Methyltransferases / metabolism Mitochondria / genetics Mitochondria / metabolism Proline / chemistry Protein Biosynthesis / genetics Protein Biosynthesis / physiology RNA / chemistry RNA / genetics RNA Processing, Post-Transcriptional / genetics* Ribosomes / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 75 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3898 |