| RRC ID |
6543
|
| 著者 |
Taira M, Sasaki M, Kimura S, Araki Y.
|
| タイトル |
Dose-dependent effects of Ni (II) ions on production of three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6), superoxide dismutase (SOD) and free radical NO by murine macrophage-like RAW264 cells with or without LPS-stimulation.
|
| ジャーナル |
J Mater Sci Mater Med
|
| Abstract |
The effect of Ni (II) ions on macrophages is not well understood. The purpose of this study was to examine the dose-dependent effects of Ni (II) ions up to 1,000 micromol/L on production of three inflammatory cytokines (TNF-alpha, IL-1beta and IL-6), superoxide dismutase (SOD) and nitric oxide (NO) by murine macrophage-like RAW264 cells with (+) or without (-) lipopolysaccharide (LPS) -stimulation. Ni (II) ions caused LPS (-) RAW264 cells to slightly increase production of TNF-alpha and IL-6, proportionally to the Ni (II) ion concentration while IL-1beta was not produced, and to slightly increase production of SOD and NO. It can be concluded that Ni (II) ions dose-dependently increased the inflammatory and oxidative stress conditions of LPS (-) RAW264 cells. LPS-stimulation caused RAW264 cells to produce in abundance the three inflammatory cytokines, SOD and NO. Ni (II) ions dose-dependently reduced the three cytokine quantities and NO amounts in LPS (+) RAW264 cells, while dose-independently increasing SOD amounts. It was noted that Ni (II) ions dose-dependently reduce the resistance power against bacteria of LPS (+) macrophages, because the production of volatile NO--bacteria killer is diminished proportionally to the Ni (II) ion concentration.
|
| 巻・号 |
19(5)
|
| ページ |
2173-8
|
| 公開日 |
2008-5-1
|
| DOI |
10.1007/s10856-007-3322-0
|
| PMID |
18040755
|
| MeSH |
Animals
Dose-Response Relationship, Drug
Free Radicals
Inflammation
Interleukin-1beta / biosynthesis*
Interleukin-6 / biosynthesis*
Ions*
Lipopolysaccharides / chemistry*
Macrophages / metabolism*
Mice
Nickel / chemistry*
Nitric Oxide / chemistry*
Nitrites / chemistry
Superoxide Dismutase / biosynthesis*
Tumor Necrosis Factor-alpha / biosynthesis*
|
| IF |
2.489
|
| 引用数 |
8
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
