論文 - 詳細
| RRC ID | 65448 |
|---|---|
| 著者 | Ganganboina AB, Takemura K, Zhang W, Li TC, Park EY. |
| タイトル | Cargo encapsulated hepatitis E virus-like particles for anti-HEV antibody detection. |
| ジャーナル | Biosens Bioelectron |
| Abstract |
Viral capsid-nanoparticle hybrid structures incorporating quantum dots (QDs) into virus-like particles (VLPs) constitute an emerging bioinspired type of nanoarchitecture paradigm used for various applications. In the present study, we packed inorganic QDs in vitro into the hepatitis E virus-like particle (HEV-LP) and developed a fluorometric biosensor for HEV antibody detection. Firstly, for the preparation of QDs-encapsulated HEV-LPs (QDs@HEV-LP), the HEV-LPs produced by a recombinant baculovirus expression system were disassembled and reassembled in the presence of QDs using the self-assembly approach. Thus, the prepared QDs@HEV-LP exhibited excellent fluorescence properties similar to QDs. Further, in the presence of HEV antibodies in the serum samples, when mixed with QDs@HEV-LP, bind together and further bind to anti-IgG-conjugated magnetic nanoparticles (MNPs). The target-specific anti-IgG-MNPs and QDs@HEV-LP enrich the HEV antibodies by magnetic separation, and the separated QDs@HEV-LP-bound HEV antibodies are quantified by fluorescence measurement. This developed method was applied to detect the HEV antibody from sera of HEV-infected monkey from 0 to 68 days-post-infection and successfully diagnosed for HEV antibodies. The viral RNA copies number from monkey fecal samples by RT-qPCR was compared to the HEV antibody generation. This study first used QDs-encapsulated VLPs as useful fluorescence emitters for biosensing platform construction. It provides an efficient route for highly sensitive and specific antibody detection in clinical diagnosis research. |
| 巻・号 | 185 |
| ページ | 113261 |
| 公開日 | 2021-8-1 |
| DOI | 10.1016/j.bios.2021.113261 |
| PII | S0956-5663(21)00298-0 |
| PMID | 33962156 |
| MeSH | Biosensing Techniques* Capsid Proteins Hepatitis Antibodies Hepatitis E* / diagnosis Hepatitis E virus* / genetics Humans |
| IF | 10.257 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sf9(RCB0563) |