論文 - 詳細
| RRC ID | 65512 |
|---|---|
| 著者 | Yamaguchi Y, Deng D, Sato Y, Hou YT, Watanabe R, Sasaki K, Kawabe M, Hirano E, Morinaga T. |
| タイトル | Silicate fiber-based 3D cell culture system for anticancer drug screening. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Three-dimensional (3D) in vitro cultures can recapitulate the physiological in vivo microenvironment. 3D Modeling techniques have been investigated and applied in anticancer drug screening. MATERIALS AND METHODS:A silicate fiber scaffold was used for 3D cell cultures, and used to model the efficacy of anticancer drugs, such as mytomicin C and doxorubicin. RESULTS:A unique 3D structure was observed in 13 human tumor cell lines on scaffold, and these cells exhibited higher drug resistance than cells in two-dimensional (2D) cultures. Furthermore, the production of lactate and expression of the nuclear factor-kappa B (NF-κB)-regulated genes B cell lymphoma-2 (BCL2), cyclooxygenase-2 (COX2), and vascular endothelial growth factor (VEGF) were higher in 3D cultures than in 2D cultures. CONCLUSION:These findings suggest that a 3D model using a silicate fiber scaffold can mimic features of cancer, and is also a suitable model for the evaluation of anticancer drugs in vitro. |
| 巻・号 | 33(12) |
| ページ | 5301-9 |
| 公開日 | 2013-12-1 |
| PII | 33/12/5301 |
| PMID | 24324063 |
| MeSH | Antineoplastic Agents / pharmacology Base Sequence Cell Culture Techniques / methods* Cell Line, Tumor Cyclooxygenase 2 / metabolism DNA Primers Doxorubicin / pharmacology Drug Screening Assays, Antitumor / methods* Gene Expression Regulation / physiology Glycolysis Humans Mitomycin / pharmacology NF-kappa B / physiology Proto-Oncogene Proteins c-bcl-2 / metabolism Real-Time Polymerase Chain Reaction Silicates* Vascular Endothelial Growth Factor A / metabolism |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | MDA-MB-453(RCB1192) DU145(RCB2143) NIH:OVCAR-3(RCB2135) |