論文 - 詳細
| RRC ID | 65515 |
|---|---|
| 著者 | Fujieda S, Sugimoto C, Seki M, Sunaga H, Saito H. |
| タイトル | CD40 stimulation inhibits cell growth and Fas-mediated apoptosis in a thyroid cancer cell line. |
| ジャーナル | Oncol Res |
| Abstract |
CD40 plays a critical role in the humoral immune response, especially in B-cell proliferation, differentiation, production of antibody, secretion of cytokines, and apoptosis. Here, we examined CD40 expression on six head and neck cancer cell lines by flow cytometry. Only the HTC/C3 cell line, which originated from a thyroid cancer, expressed CD40 on the surface of the cells. Coculture with anti-CD40 mAb inhibited colony formation of HTC/C3 cells. CD40 stimulation enhanced Fas expression on HTC/C3 cells. Although HTC/C3 cells are sensitive to Fas-mediated apoptosis, CD40 stimulation inhibited Fas-mediated apoptosis in HTC/C3 cells. CD40 stimulation enhanced Bcl-2 expression, and antisense oligonucleotide against bcl-2 canceled the inhibition of HTC/C3 cell growth caused by CD40 stimulation. Additionally, more anti-CD40 mAb-treated HTC/C3 cells were accumulated in G1 phase, and fewer in S phase, compared to nontreated cells. These results suggest that CD40 stimulation might be involved in the slow growth rate of CD40-bearing cancer cells, and they suggest a new biological approach to the treatment of cancers. |
| 巻・号 | 10(9) |
| ページ | 433-9 |
| 公開日 | 1998-1-1 |
| PMID | 10223618 |
| MeSH | Antibodies, Monoclonal / pharmacology Apoptosis / drug effects Apoptosis / physiology* CD40 Antigens / drug effects CD40 Antigens / immunology CD40 Antigens / metabolism* Carcinoma / drug therapy Carcinoma / metabolism Carcinoma / pathology* Cell Division / drug effects Cell Division / physiology G1 Phase / drug effects Head and Neck Neoplasms / drug therapy Head and Neck Neoplasms / metabolism Head and Neck Neoplasms / pathology Humans Oligonucleotides, Antisense / pharmacology Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism Signal Transduction Thyroid Neoplasms / drug therapy Thyroid Neoplasms / metabolism Thyroid Neoplasms / pathology* Tumor Cells, Cultured / drug effects Tumor Cells, Cultured / metabolism Tumor Stem Cell Assay fas Receptor / metabolism* |
| IF | 4.949 |
| リソース情報 | |
| ヒト・動物細胞 | HTC/C3(RCB0452) |