論文 - 詳細
| RRC ID | 65527 |
|---|---|
| 著者 | Shim I, Choi K, Hirano S. |
| タイトル | Oxidative stress and cytotoxic effects of silver ion in mouse lung macrophages J774.1 cells. |
| ジャーナル | J Appl Toxicol |
| Abstract |
Silver is commonly used as a disinfectant, and chronic exposure to silver may cause argyria, resulting in a gray-blue discoloration of human skin. However, the mechanism for cellular toxicity of silver has not been well explained. We studied the mode of cell death, the ratio of glutathione disulfide/glutathione, induction of metallothionein and activation of mitogen-activated protein kinases in J774.1 cells together with activation of antioxidant responsive element and nuclear factor-κB in CHO cells following exposure to silver ion (Ag+ ) to investigate the mechanism by which Ag+ causes lethal effects. Ag+ increased phosphorylation levels of extracellular signal-regulated, c-Jun N-terminal and p38 mitogen-activated protein kinases and remarkably increased the ratio of glutathione disulfide/glutathione in both a time- and concentration-dependent manner. Luciferase reporter gene assays revealed that antioxidant responsive element and nuclear factor-κB were activated following exposure to Ag+ . In addition, exposure to Ag+ increased the mRNA and protein levels of metallothionein. We investigated whether or not Ag+ killed J774.1 cells by inducing apoptosis. Ag+ increased the activity of caspase-3/7 which was abrogated by caspase 3 and pan-caspase inhibitors. However, these inhibitors did not ameliorate the cytotoxic effects of Ag+ , suggesting that Ag+ causes oxidative stress, which leads to necrotic rather than apoptotic cell death in J774.1 cells by decreasing functional sulfhydryl groups including glutathione in the cells. Copyright © 2016 John Wiley & Sons, Ltd. |
| 巻・号 | 37(4) |
| ページ | 471-478 |
| 公開日 | 2017-4-1 |
| DOI | 10.1002/jat.3382 |
| PMID | 27624343 |
| MeSH | Animals Apoptosis / drug effects CHO Cells Caspase Inhibitors / pharmacology Caspases / biosynthesis Caspases / genetics Cell Line Cell Survival / drug effects Cricetulus Genes, Reporter / drug effects Glutathione / metabolism Lung / cytology* Lung / drug effects Macrophages / drug effects* Metallothionein / metabolism Mice Necrosis Oxidative Stress / drug effects* Silver / chemistry Silver / toxicity* |
| IF | 2.997 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | J774.1 CHO-K1(RCB0285) |