RRC ID 65602
Author Kuwano T, Izumi H, Aslam MR, Igarashi Y, Bilal M, Nishimura A, Watanabe Y, Nawaz A, Kado T, Ikuta K, Yamamoto S, Sasahara M, Fujisaka S, Yagi K, Mori H, Tobe K.
Title Generation and characterization of a Meflin-CreERT2 transgenic line for lineage tracing in white adipose tissue.
Journal PLoS One
Abstract Meflin (Islr) expression has gained attention as a marker for mesenchymal stem cells, but its function remains largely unexplored. Here, we report the generation of Meflin-CreERT2 mice with CreERT2 inserted under the Meflin gene promoter to label Meflin-expressing cells genetically, thereby enabling their lineages to be traced. We found that in adult mice, Meflin-expressing lineage cells were present in adipose tissue stroma and had differentiated into mature adipocytes. These cells constituted Crown-like structures in the adipose tissue of mice after high-fat diet loading. Cold stimulation led to the differentiation of Meflin-expressing lineage cells into beige adipocytes. Thus, the Meflin-CreERT2 mouse line is a useful new tool for visualizing and tracking the lineage of Meflin-expressing cells.
Volume 16(3)
Pages e0248267
Published 2021-1-1
DOI 10.1371/journal.pone.0248267
PII PONE-D-20-35143
PMID 33760832
PMC PMC7990287
MeSH Adipose Tissue, White* / cytology Adipose Tissue, White* / metabolism Animals Cell Differentiation Cell Lineage Gene Expression Immunoglobulins* / genetics Immunoglobulins* / metabolism Mesenchymal Stem Cells / cytology* Mice Mice, Inbred C57BL Mice, Transgenic*
IF 2.74
DNA material B6N Mouse BAC clone (RDB07573) B6Ng01-165L10P